SLC25A12 Antibody (JE65-52)

Western Blot: SLC25A12 Antibody (JE65-52) [NBP3-32952] - Western blot analysis of SLC25A12 on different lysates with Rabbit anti-SLC25A12 antibody (NBP3-32952) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Raji cell lysate Lane 3: RAW264.7 cell lysate Lane 4: Rat kidney tissue lysate (20 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 75 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32952) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: SLC25A12 Antibody (JE65-52) [NBP3-32952] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SLC25A12 antibody (NBP3-32952) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32952) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: JE65-52
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SLC25A12 Antibody (JE65-52) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within human SLC25A12 aa 601-650/678. (Uniprot: O75746)
• Localization: Mitochondrion inner membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SLC25A12
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:500
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SLC25A12 Antibody (JE65-52)

• AGC1
• araceli hiperlarga
• ARALAR
• ARALAR1
• calcium binding mitochondrial carrier superfamily member Aralar1
• calcium-binding mitochondrial carrier protein Aralar1
• Mitochondrial aspartate glutamate carrier 1
• solute carrier family 25 (mitochondrial carrier, Aralar), member 12
• Solute carrier family 25 member 12

Background

The SLC25A12 gene encodes a calcium-binding mitochondrial carrier protein. The encoded protein localizes to the mitochondria and is involved in the exchange of aspartate for glutamate across the inner mitochondrial membrane. Polymorphisms in the gene encoding thi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC25A12