Human ADAMTS5 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human ADAMTS5 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ADAMTS5

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human ADAMTS5 DuoSet ELISA, 5 Plate

• A disintegrin and metalloproteinase with thrombospondin motifs 11
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 5 (aggrecanase-2)
• ADAM metallopeptidase with thrombospondin type 1 motif, 5
• ADAM-TS 11
• ADAM-TS 5
• ADAMTS11
• ADAMTS-11
• ADAMTS5
• ADAM-TS5
• ADAMTS-5
• ADMP2
• ADMP-2
• ADMP-2A disintegrin and metalloproteinase with thrombospondin motifs 5
• Aggrecanase 2
• aggrecanase-2
• disintegrin-like and metalloprotease with thrombospondin type 1 motif, 510ADAMTS11FLJ36738
• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.14
• EC 3.4.24.82

Background

Members of the ADAMTS family contain pro, catalytic, disintegrin-like, cysteine-rich, spacer and a variable number of TS type 1 motifs. In contrast to membrane-anchored ADAMs, ADAMTSs are secreted and are all active proteases. They are essential for aggrecan turnover and procollagen processing, regulation of which is important in many physiological and pathological processes such as angiogenesis and arthritis.

Publications for ADAMTS5 (DY2198-05)(16)

Publications using DY2198-05

• Sengprasert, P;Waitayangkoon, P;Kamenkit, O;Sawatpanich, A;Chaichana, T;Wongphoom, J;Ngarmukos, S;Taweevisit, M;Lotinun, S;Tumwasorn, S;Tanavalee, A;Reantragoon, R; Catabolic mediators from TLR2-mediated proteoglycan aggrecan peptide-stimulated chondrocytes are reduced by Lactobacillus-conditioned media Scientific reports 2024-08-05 [PMID: 39103466] (Human)
• Sun, R;Zhu, J;Sun, K;Gao, L;Zheng, B;Shi, J; Strontium Ranelate Ameliorates Intervertebral Disc Degeneration via Regulating TGF-?1/NF-?B Axis International journal of medical sciences 2023-10-07 [PMID: 37928874] (Human)
• X Wang, D Wang, B Deng, L Yan Syringaresinol attenuates osteoarthritis via regulating the NF-kappaB pathway International immunopharmacology, 2023-03-28;118(0):109982. 2023-03-28 [PMID: 36989902] (Mouse)
• Z Zhou, L Zhang, Y Liu, C Huang, W Xia, H Zhou, Z Zhou, X Zhou Luteolin Protects Chondrocytes from H2O2-Induced Oxidative Injury and Attenuates Osteoarthritis Progression by Activating AMPK-Nrf2 Signaling Oxidative Medicine and Cellular Longevity, 2022-02-01;2022(0):5635797. 2022-02-01 [PMID: 35154568] (Mouse)
• DF Boyd, EK Allen, AG Randolph, XJ Guo, Y Weng, CJ Sanders, R Bajrachary, NK Lee, CS Guy, P Vogel, W Guan, Y Li, X Liu, T Novak, MM Newhams, TP Fabrizio, N Wohlgemuth, PM Mourani, PALISI Ped, TN Wight, S Schultz-Ch, SA Cormier, K Shaw-Salib, A Pekosz, RE Rothman, KF Chen, Z Yang, RJ Webby, N Zhong, JC Crawford, PG Thomas Exuberant fibroblast activity compromises lung function via ADAMTS4 Nature, 2020-10-28;0(0):. 2020-10-28 [PMID: 33116313] (Human)
• J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants Bone Rep, 2020-09-11;13(0):100715. 2020-09-11 [PMID: 32995386] (Human)
• Z Zhang, Z Zha, Z Zhao, W Liu, W Li Lentinan Inhibits AGE-Induced Inflammation and the Expression of Matrix-Degrading Enzymes in Human Chondrocytes Drug Des Devel Ther, 2020-07-17;14(0):2819-2829. 2020-07-17 [PMID: 32764881] (Human)
• R Liu, H Wu, H Song Knockdown of TRIM8 Attenuates IL-1beta-induced Inflammatory Response in Osteoarthritis Chondrocytes Through the Inactivation of NF-kappaB Pathway Cell Transplant, 2020-01-01;29(0):9636897209436. 2020-01-01 [PMID: 32757662] (Human)
• XH Xue, JX Xue, W Hu, FL Shi, Y Yang Nomilin targets the Keap1-Nrf2 signalling and ameliorates the development of osteoarthritis J. Cell. Mol. Med., 2020-06-21;0(0):. 2020-06-21 [PMID: 32564468] (Mouse)
• J Lee, CY Lee, JH Park, HH Seo, S Shin, BW Song, IK Kim, SW Kim, S Lee, JC Park, S Lim, KC Hwang Differentiation of adipose-derived stem cells into functional chondrocytes by a small molecule that induces Sox9 Exp. Mol. Med., 2020-04-21;0(0):. 2020-04-21 [PMID: 32313200] (Human)
• Z Wang, D Ye, J Ye, M Wang, J Liu, H Jiang, Y Xu, J Zhang, J Chen, J Wan ADAMTS-5 Decreases in Coronary Arteries and Plasma from Patients with Coronary Artery Disease Dis. Markers, 2019-12-16;2019(0):6129748. 2019-12-16 [PMID: 31929842] (Human)
• D Chen, D Lu, H Liu, E Xue, Y Zhang, P Shang, X Pan Pharmacological blockade of PCAF ameliorates osteoarthritis development via dual inhibition of TNF-alpha-driven inflammation and ER stress EBioMedicine, 2019-11-14;0(0):. 2019-11-14 [PMID: 31735552] (Human)
• X Li, J Lin, X Ding, J Xuan, Z Hu, D Wu, X Zhu, Z Feng, W Ni, A Wu The protective effect of sinapic acid in osteoarthritis: In vitro and in vivo studies J. Cell. Mol. Med., 2019-01-03;0(0):. 2019-01-03 [PMID: 30604480] (Human)
• X Li, D Wu, Z Hu, J Xuan, X Ding, G Zheng, Z Feng, W Ni, A Wu The Protective Effect of Ligustilide in Osteoarthritis: An in Vitro and in Vivo Study Cell. Physiol. Biochem., 2018-08-17;48(6):2583-2595. 2018-08-17 [PMID: 30121673] (Human)
• MH Hwang, HG Son, JW Lee, CM Yoo, JH Shin, HG Nam, HJ Lim, SM Baek, JH Park, JH Kim, H Choi Photobiomodulation of extracellular matrix enzymes in human nucleus pulposus cells as a potential treatment for intervertebral disk degeneration Sci Rep, 2018-08-03;8(1):11654. 2018-08-03 [PMID: 30076336] (Human)
• DS Mern, A Tschugg, S Hartmann, C Thomé Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders PLoS ONE, 2017-02-16;12(2):e0172181. 2017-02-16 [PMID: 28207788] (Human)

FAQs for ADAMTS5 (DY2198-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ADAMTS5