SLC1A5 Antibody (1085750) [Unconjugated]

HAF018). A specific band was detected for SLC1A5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." > HAF018). A specific band was detected for SLC1A5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." title="Western Blot shows lysates of HCT‑116 human colorectal carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human SLC1A5 Monoclonal Antibody (Catalog # MAB11603) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SLC1A5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." />

Western Blot shows lysates of HCT‑116 human colorectal carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human SLC1A5 Monoclonal Antibody (Catalog # MAB11603) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="SLC1A5 was detected in immersion fixed RT-4 human bladder carcinoma cell line (Positive) and SW480 human colorectal adenocarcinoma cell line (Positive) using Mouse Anti-Human SLC1A5 Monoclonal Antibody (Catalog # mab11603) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

SLC1A5 was detected in immersion fixed RT-4 human bladder carcinoma cell line (Positive) and SW480 human colorectal adenocarcinoma cell line (Positive) using Mouse Anti-Human SLC1A5 Monoclonal Antibody (Catalog # mab11603) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of glandular cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." > VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of glandular cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." title="SLC1A5 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human SLC1A5 Monoclonal Antibody (Catalog # mab11603) at 0.1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of glandular cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." />

SLC1A5 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human SLC1A5 Monoclonal Antibody (Catalog # mab11603) at 0.1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 1085750
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SLC1A5 Antibody (1085750) [Unconjugated] Summary

• Immunogen: Synthetic Peptide
  Accession # Q15758
• Specificity: Detects a synthetic peptide corresponding to residues near amino acid 500 of human SLC1A5 protein by Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 0.1-10 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLC1A5 Antibody (1085750) [Unconjugated]

• AAAT
• Alanine/serine/cysteine transporter 2
• ASC amino acid transporter-2
• ASCT2
• ATB(0)
• ATBO
• baboon M7 virus receptor
• M7V1
• M7VS1
• neutral amino acid transporter B
• neutral amino acid transporter B(0)
• R16
• RD114 virus receptor
• RD114/simian type D retrovirus receptor
• RDR
• RDRC
• SLC1A5
• Sodium-dependent neutral amino acid transporter type 2
• solute carrier family 1 (neutral amino acid transporter), member 5
• Solute carrier family 1 member 5

Background

SLC1A5 is a sodium dependent antiporter also known as ASCT2 (ASC amino acid Transporter2).  It is a member of the solute carrier (SLC) superfamily of transporters and plays an important role in tumors.  It is one of the 3 proteins, including SLC7A5 and SLC3A2, that are among the highest differentially expressed genes in activated lymphocytes and cancerous cells.  Pharmacological inhibition of SLC1A5 was found to abrogate the effector functions of NK cells.  In a pancancer analysis of SLC1A5, it has been demonstrated to be a prognostic biomarker in cancer patients.

1. Nachef M, Ali AK, Almutairi SM, Lee SH. Targeting SLC1A5 and SLC3A2/SLC7A5 as a Potential Strategy to Strengthen Anti-Tumor Immunity in the Tumor Microenvironment. Front Immunol. 2021 Apr 19;12:624324. doi: 10.3389/fimmu.2021.624324. PMID: 33953707; PMCID: PMC8089370. 
2. Chen P, Jiang Y, Liang J, Cai J, Zhuo Y, Fan H, Yuan R, Cheng S, Zhang Y. SLC1A5 is a novel biomarker associated with ferroptosis and the tumor microenvironment: a pancancer analysis. Aging (Albany NY). 2023 Aug 10;15(15):7451-7475. doi: 10.18632/aging.204911. Epub 2023 Aug 10. PMID: 37566748; PMCID: PMC10457057.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q15758(Human)