Human RBP3 ELISA Kit (Colorimetric)

ELISA: Human RBP3 ELISA Kit (Colorimetric) [NBP2-82525] - Samples were spiked with high concentrations of Human RBP3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human RBP3 ELISA Kit (Colorimetric) [NBP2-82525] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Human RBP3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human RBP3 in samples. No Significant cross-reactivity or interference between Human RBP3 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.06
• Intra-Assay: CV% < 4.68
• Spike Recovery: 85-107%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: RBP3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human RBP3 ELISA Kit (Colorimetric)

• D10S64
• D10S65
• D10S66
• Interphotoreceptor retinoid-binding protein
• Interstitial retinol-binding protein
• IRBP
• RBPI
• retinol binding protein 3, interstitial
• retinol-binding protein 3
• retinol-binding protein 3, interstitial

Background

RBP3, also known as Retinol-binding protein 3, is a secreted 135 kDa 1247 amino acid protein. RBP3 plays a critical role in the visual process by transporting retinoids between the retinal pigment epithelium and the photoreceptors. RBP3 is currently being studied in relationship to retinitis, retinoblastoma, Behcet's disease, diphtheria, night blindness and proliferative vitreoretinopathy. RBP3 is linked to the g1phase, cell cycle, induction of apoptosis, visual cycle I and cell cycle arrest pathways where it interacts with MCPH1, TFDP1, TFDP2, NOTCH1 and RB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for RBP3 ELISA Kit (NBP2-82525). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: RBP3