HERV Antibody

Western Blot: HERV Antibody [NB100-93579] - WB detection of HERV in human placenta lysate with NB100-93579 antibody at a dilution of 1:250

• Reactivity: Hu
• Applications: WB, IHC, IHC-Fr, IHC-P, In vitro, B/N
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

HERV Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues 42-50 [QRPGNIDAPC] of the HERV protein.
• Specificity: Human Endogenous Retroviruses (HERVs), a class of multiple-sclerosis-associated retroviruses
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERVW-1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:250-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • In vitro assay
  • Block/Neutralize
• Application Notes: This antibody is useful for Western Blot. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24635849). Use in In vitro, Immunohistochemistry-Frozen and blocking/neutralizing reported in scientific literature (PMID 26154387) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 59.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HERV Antibody

• endogenous retroviral family W, env(C7), member 1
• ENV
• envelope glycoprotein
• Envelope polyprotein gPr73
• enverin
• ENVW
• Env-W
• HERV-7q Envelope protein
• HERV7Q
• HERV-7q
• HERV-tryptophan envelope protein
• HERV-W Env glycoprotein
• HERV-W envelope protein
• HERV-W
• HERV-W_7q21.2 provirus ancestral Env polyprotein
• HERV-W{7q21.1} provirus ancestral Env polyprotein
• HERVWENV
• HERV-W-ENV
• HERVWenvelope protein
• human endogenous retrovirus W envC7-1 envelope protein
• syncytin A
• Syncytin
• syncytin-1

Background

HERVs are a class of multiple sclerosis associated retroviruses. Reacts with a replication competent Human Endogenous Retrovirus (HERVW) isolated from placenta. The synthetic peptide sequence used as immunogen is taken from the virus envelope polyprotein sequence. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. The HERVW endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HERV Antibody (NB100-93579)(2)

Publications using NB100-93579

• Frese S, Ruebner M, Suhr F et al. Long-Term Endurance Exercise in Humans Stimulates Cell Fusion of Myoblasts along with Fusogenic Endogenous Retroviral Genes In Vivo PLoS ONE. 2015 Jul 09 [PMID: 26154387] (In vitro, B/N, IHC-Fr, Human)
  
  Details:
  HERV / Syncytin-1 antibody was used at 1:200 dilution for IHC staining of human muscle cryosections and normal human placental control tissues (third trimester) were employed as control for the staining evaluation (Fig 5). Moreover, the antibody was used for in-vitro blocking/neutrilization experiments involving cultured primary human myoblasts (0.5 - 1.0 ug/ml concentration; data in Fig 7, S1).
• Buslei R, Strissel PL, Henke C et al. Activation and regulation of endogenous retroviral genes in the human pituitary gland and related endocrine tumors. Neuropathol. Appl. Neurobiol. 2014 Mar 17 [PMID: 24635849] (IHC-P, Human)
  
  Details:
  IHC: Normal pituitary adenohypophysis and pituitary adenomas, Figs 1,2; WB: primary cultures of pituitary adenomas, Fig 5A.

Review for HERV Antibody (NB100-93579) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Greta Beckerle ICC Human 11/15/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Breast cancer cells
• Species: Human
• Lot: 1920

Comments

• Comments: TEM imaging of T47D and MCF7 breast cancer cells.

FAQs for HERV Antibody (NB100-93579). (Showing 1 - 1 of 1 FAQs).

1. Does the antibody recognize the HERV-W HYPER product, the digest result of the fool GENE PRODUCT HERV-W?
   • HERV Antibody [NB100-93579] is generated against immunogen sequence QRPGNIDAPC which shares sequence homology with your mentioned sequence which was..... FTLTAPPPCRCMTSSSPYQEFLWRMQRPGNIDAPSY...... Therefore, we strongly believe that our antibody NB100-93579 will be cross-reacting with your mentioned partial protein.

Bioinformatics

• Gene Symbol: ERVW-1
• Entrez:
  • 30816(Human)
• Uniprot:
  • Q9UQF0(Human)