SLC38A1 Antibody (S104-32)

Western Blot: SLC38A1 Antibody (S104-32) [NBP2-59311] - Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32 (NBP2-59311). Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody (NBP2-59311) at 1:1000.

Immunocytochemistry/Immunofluorescence: SLC38A1 Antibody (S104-32) [NBP2-59311] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SNAT1 Monoclonal Antibody, Clone S104-32 (NBP2-59311). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody (NBP2-59311) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SNAT1 Antibody (D) Composite.

Western Blot: SLC38A1 Antibody (S104-32) [NBP2-59311] - Analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32. Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody at 1:1000.

Immunocytochemistry/Immunofluorescence: SLC38A1 Antibody (S104-32) [NBP2-59311] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SNAT1 Antibody (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S104-32
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SLC38A1 Antibody (S104-32) Summary

• Immunogen: Fusion protein amino acids 1- 63 of rat SNAT1
• Localization: Cell Membrane
• Specificity: Detects 50kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC38A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for SLC38A1 Antibody (S104-32)

• Amino acid transporter A1
• ATA1SNAT1
• NAT2N-system amino acid transporter 2
• SAT1amino acid transporter system A1
• sodium-coupled neutral amino acid transporter 1
• Solute carrier family 38 member 1
• solute carrier family 38, member 1
• System A amino acid transporter 1
• System N amino acid transporter 1

Background

Amino acid transporters play essential roles in the uptake of nutrients, production of energy, chemical metabolism,detoxification, and neurotransmitter cycling. SLC38A1 is an important transporter of glutamine, an intermediate in thedetoxification of ammonia and the production of urea. Glutamine serves as a precursor for the synaptic transmitter,glutamate (Gu et al., 2001 (PubMed 11325958)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC38A1 Antibody (NBP2-59311)(1)

Publication using NBP2-59311

• Davidson-Hunt A Supplemental glutamine to restore glutathione homeostasis in the aging mouse liver Thesis 2022-01-01 (WB, Mouse)
  
  Details:
  Dilution used in WB 1:500

Bioinformatics

• Gene Symbol: SLC38A1