Sirtuin 3/SIRT3 Antibody - BSA Free

Western Blot: Sirtuin 3/SIRT3 Antibody - BSA Free [NBP1-76264] - Western blot analysis of Sirtuin 3/SIRT3 in mouse heart tissue lysate with Sirtuin 3/SIRT3 antibody at (A) 1 and (B) 2 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Sirtuin 3/SIRT3 Antibody - BSA Free Summary

• Description: Novus Biologicals Chicken Sirtuin 3/SIRT3 Antibody - BSA Free (NBP1-76264) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the center of human SIRT3. The immunogen is located within amino acids 180 - 230 of SIRT3. Amino Acid Squence: LAKELYPGNYKPNVTHY
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: SIRT3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1-2 ug/ml
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Sirtuin 3/SIRT3 Protein (NBP1-76264PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Sirtuin 3/SIRT3 Antibody - BSA Free

• EC 3.5.1
• EC 3.5.1.-
• hSIRT3
• mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
• NAD-dependent deacetylase sirtuin-3, mitochondrial
• SIR2L3
• SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
• sir2-like 3
• SIR2-like protein 3
• SIRT3
• sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
• Sirtuin 3
• sirtuin type 3

Background

The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence, lifespan extension and several age-related diseases. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT3
• Entrez:
  • 23410(Human)
  • 64384(Mouse)
  • 293615(Rat)
• Uniprot:
  • Q9NTG7(Human)