Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10)

Immunocytochemistry/Immunofluorescence: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometry: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878] - Flow cytometric analysis of PFA-fixed HeLa cells. Sirtuin 3/SIRT3 antibody (PCRP-SIRT3-1C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-SIRT3-1C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14289)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human Sirtuin 3/SIRT3 protein (Uniprot: Q9NTG7)
• Localization: Mitochondrion matrix
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRT3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 43.57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10)

• EC 3.5.1
• EC 3.5.1.-
• hSIRT3
• mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
• NAD-dependent deacetylase sirtuin-3, mitochondrial
• SIR2L3
• SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
• sir2-like 3
• SIR2-like protein 3
• SIRT3
• sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
• Sirtuin 3
• sirtuin type 3

Background

SIRT3 is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT3
• Uniprot:
  • Q9NTG7(Human)