Sirtuin 1/SIRT1 Antibody (SZ04-01)

Western Blot: Sirtuin 1/SIRT1 Antibody (SZ04-01) [NBP2-67364] - Western blot analysis of Sirtuin 1/SIRT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goa

Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (SZ04-01) [NBP2-67364] - Staining SIRT1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (SZ04-01) [NBP2-67364] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Sirtuin 1/SIRT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-3, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Sirtuin 1/SIRT1 Antibody (SZ04-01) [NBP2-67364] - Analysis of SIRT1 on Jurkat cells lysates using anti-SIRT1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (SZ04-01) [NBP2-67364] - Staining SIRT1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (SZ04-01) [NBP2-67364] - Staining SIRT1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Sirtuin 1/SIRT1 Antibody (SZ04-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Sirtuin 1/SIRT1 aa 698-747 / 747. (SwissProt: Q96EB6 Human; SwissProt: Q923E4 Mouse)
• Localization: Nucleus, Cytoplasm, Mitochondrion.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SIRT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:1000
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Sirtuin 1/SIRT1 Antibody (SZ04-01)

• EC 3.5.1
• S. cerevisiae, homolog) 1
• SIR2alpha
• SIR2L1
• SIR2-like protein 1
• SIRT1
• Sirtuin 1

Background

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2 , SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT1