Sirtuin 1/SIRT1 Antibody (7B7) - Azide and BSA Free

Western Blot: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - SIRT1 monoclonal antibody (M01), clone 7B7 Analysis of SIRT1 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Analysis of monoclonal antibody to SIRT1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Analysis of monoclonal antibody to SIRT1 on formalin-fixed paraffin-embedded human lateral ventricle wall. Antibody concentration 1 ug/ml.

Sandwich ELISA: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Detection limit for recombinant GST tagged SIRT1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 7B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Sirtuin 1/SIRT1 Antibody (7B7) - Azide and BSA Free Summary

• Immunogen: SIRT1 (AAH12499, 456 a.a. ~ 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS
• Localization: Nuclear
• Specificity: SIRT1 - sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Sirtuin 1/SIRT1 Antibody (7B7) - Azide and BSA Free

• EC 3.5.1
• S. cerevisiae, homolog) 1
• SIR2alpha
• SIR2L1
• SIR2-like protein 1
• SIRT1
• Sirtuin 1

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sirtuin 1/SIRT1 Antibody (H00023411-M01)(4)

Publications using H00023411-M01

• Conrad E, Polonio-Vallon T, Meister M et al. HIPK2 restricts SIRT1 activity upon severe DNA damage by a phosphorylation-controlled mechanism Cell Death Differ. 2015-06-26 [PMID: 26113041] (ICC/IF, Human)
• Kok SH, Lin LD, Hou KL et al. Simvastatin inhibits Cyr61 expression in rheumatoid arthritis synovial fibroblasts through the regulation of SIRT1/FoxO3a signaling. Arthritis Rheum 2012-12-12 [PMID: 23239110]
• Ostrup O, Hyttel P, Klarke DA, Collas P. Remodeling of ribosomal genes in somatic cells by Xenopus egg extract. Biochem Biophys Res Commun. 2011-08-06 [PMID: 21843509]
• Hiraike H, Wada-Hiraike O, Nakagawa S et al. Identification of DBC1 as a transcriptional repressor for BRCA1. Br J Cancer 102(6):1061-7. 2010-03-16 [PMID: 20160719]

Bioinformatics

• Gene Symbol: SIRT1
• Entrez:
  • 23411(Human)
• OMIM:
  • 604479(Human)
• Uniprot:
  • AAH12499(Human)