SIRP gamma/CD172g Antibody (595337)

Western blot shows lysates of NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SIRP gamma/CD172g Monoclonal Antibody (Catalog # MAB4486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SIRP gamma/CD172g at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 595337
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SIRP gamma/CD172g Antibody (595337) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human SIRP gamma /CD172g
  Val64-Ser364
  Accession # Q9P1W8
• Specificity: Detects human SIRP gamma /CD172g in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 20% cross-reactivity with recombinant human (rh) SIRP alpha and rhSIRP beta 1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRPG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIRP gamma/CD172g Antibody (595337)

• bA77C3.1
• CD172g antigen
• CD172g
• FLJ42230
• signal-regulatory protein beta 2
• Signal-regulatory protein beta-2
• signal-regulatory protein gamma
• SIRP beta 2
• SIRP gamma
• SIRP-B2
• SIRPB2CD172 antigen-like family member B
• SIRP-beta-2
• SIRPG
• SIRPgamma
• SIRP-gamma

Background

Signal-regulatory protein gamma/beta 2 (SIRP gamma/beta 2) is a 45-47 kDa type 1 transmembrane glycoprotein and member of the immunoglobulin superfamily and SIRP family of proteins. Human SIRP gamma/beta 2 is synthesized as a 387 amino acid (aa) precursor that contains a 28 aa signal sequence, a 332 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 4 aa cytoplasmic tail. The ECD contains two Ig-like C1-type domains, one Ig-like V-type domain, and four potential sites for N-linked glycosylation. There are four named isoforms for human SIRP gamma/beta 2. Human SIRP gamma/beta 2 has no orthologs, but it is 75% aa identical to SIRP beta . It is expressed in the liver and at very low levels in brain, heart, lung, pancreas, kidney, placenta, and skeletal muscle. SIRP gamma/beta 2 is also expressed on CD4⁺ T-cells, CD8⁺ T-cells, and activated NK cells. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRPG
• Uniprot:
  • Q9P1W8()