MKP-1/DUSP1 Antibody (JJ0930)

Images

 
Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of MKP-1/DUSP1 on SK-BR-3 cell tissue lysates with Rabbit anti-MKP-1/DUSP1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band ...read more
Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of paraffin-embedded mouse lung tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.
Flow Cytometry: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of HepG2 cells with DUSP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; ...read more
Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of DUSP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary ...read more
Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of paraffin-embedded human lung cancer tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Product Discontinued
View other related MKP-1/DUSP1 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-67909
    • Availability
      Product Discontinued

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MKP-1/DUSP1 Antibody (JJ0930) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human MKP-1/DUSP1 aa 112-156 / 367. (SwissProt: P28562 Human; SwissProt: P28563 Mouse; SwissProt: Q64623 Rat)
Localization
Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
DUSP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
Publications
Read Publications using
NBP2-67909 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for MKP-1/DUSP1 Antibody (JJ0930)

  • CL100
  • CL100MAP kinase phosphatase 1
  • dual specificity phosphatase 1
  • Dual specificity protein phosphatase hVH1
  • DUSP1
  • EC 3.1.3.16
  • EC 3.1.3.48
  • HVH1
  • Mitogen-activated protein kinase phosphatase 1
  • MKP1
  • MKP-1
  • MKP-1dual specificity protein phosphatase 1
  • Protein-tyrosine phosphatase CL100
  • PTPN10
  • PTPN10serine/threonine specific protein phosphatase
  • VH1

Background

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MKP-1/DUSP1 Antibody (NBP2-67909)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DUSP1