L1CAM Antibody (UJ127.11)

Images

 
Western Blot: L1CAM Antibody (UJ127.11) [NB100-2682] - SUM159 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. L1CAM Antibody (NB100-2682) primary antibody: ...read more
Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (UJ127.11) [NB100-2682] - The left panel (A) shows untreated Neuro2a cells and the right panel (B) shows Neuro2a cells that were serum starved then treated with ...read more
Immunohistochemistry-Frozen: L1CAM Antibody (UJ127.11) [NB100-2682] - Human pancreatic ductal adenocarcinoma (PDAC) tissue section stained with L1CAM antibody. IHC-Fr image submitted by a verified customer review.
Flow Cytometry: L1CAM Antibody (UJ127.11) [NB100-2682] - A surface stain was performed on HeLa cells with L1CAM Antibody (UJ127.11) NB100-2682 (blue) and a matched isotype control (orange). Cells were incubated in an ...read more
Western Blot: L1CAM Antibody (UJ127.11) [NB100-2682] - Total protein from human HeLa, A431 and SHSY-5Y cell lines and human Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (UJ127.11) [NB100-2682] - The left panel (A) shows untreated Neuro2a cells and the right panel (B) shows Neuro2a cells that were serum starved then treated with ...read more
Immunohistochemistry-Paraffin: L1CAM Antibody (UJ127.11) [NB100-2682] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of L1CAM [UJ127.11] antibody. The staining was developed using HRP labeled ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, ICC/IF
Clone
UJ127.11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

L1CAM Antibody (UJ127.11) Summary

Immunogen
Homogenous suspension of 16 week human foetal brain.
Localization
Cell Membrane
Marker
Axon Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
L1CAM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 2.5 ug/mL
  • Immunocytochemistry/Immunofluorescence 2 - 5 ug/mL
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
  • Flow (Cell Surface) 2.5 ug/mL
  • Immunofluorescence 1 - 2 ug/mL
Application Notes
This antibody is CyTOF ready.
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-2682 in the following applications:

Publications
Read Publications using
NB100-2682 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Notes

Positive control(s): Frozen mouse E16.5 lung sections (IHC), Cell lines TR 14, LAN-1, CHP 100, CHP212, CHP126 ( Neuroblastomas or Schwannomas) (IF), M5 melanoma cells or human foetal brain (WB).

Alternate Names for L1CAM Antibody (UJ127.11)

  • antigen identified by monoclonal R1
  • CAML1
  • CAML1N-CAML1
  • CD171 antigen
  • CD171
  • HSAS
  • HSAS1
  • L1 cell adhesion molecule
  • L1CAM
  • MASA
  • MIC5
  • N-CAM-L1
  • NCAM-L1
  • neural cell adhesion molecule L1
  • S10
  • SPG1

Background

The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for L1CAM Antibody (NB100-2682)(2)

Reviews for L1CAM Antibody (NB100-2682) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-2682:
Filter by Applications
IHC-Fr
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen L1CAM NB100-2682
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Human 08/13/2021
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedpancreatic ductal adenocarcinoma (PDAC)
SpeciesHuman
LotUJ127.11
Western Blot L1CAM NB100-2682
Enlarge
5
reviewed by:
Verified Customer
WB Human 11/04/2020
View

Summary

ApplicationWestern Blot
Sample TestedSUM-159PT
SpeciesHuman
LotCMSD02-2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for L1CAM Antibody (NB100-2682) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2682AF350
Alexa Fluor 405 NB100-2682AF405
Alexa Fluor 488 NB100-2682AF488
Alexa Fluor 532 NB100-2682AF532
Alexa Fluor 594 NB100-2682AF594
Alexa Fluor 647 NB100-2682AF647
Alexa Fluor 700 NB100-2682AF700
Alexa Fluor 750 NB100-2682AF750
Allophycocyanin/Cy7 NB100-2682APCCY7
Biotin NB100-2682B
DyLight 350 NB100-2682UV
DyLight 405 NB100-2682V
DyLight 488 NB100-2682G
DyLight 550 NB100-2682R
DyLight 594 NB100-2682DL594
DyLight 650 NB100-2682C
DyLight 680 NB100-2682FR
DyLight 755 NB100-2682IR
FITC NB100-2682F
HRP NB100-2682H
Janelia Fluor 549 NB100-2682JF549
Janelia Fluor 646 NB100-2682JF646
PE/Atto594 NB100-2682PEATT594
PE/Cy5.5 NB100-2682PECY55
PE/Cy7 NB100-2682PECY7

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Blogs on L1CAM.

Identifying tumoral and stromal transcriptomes that underlie tumor plasticity and stromal neuroinflammatory response in brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Cancers in the brain often come from tumors elsewhere in the body. Several adaptive mechanisms influence brain metastasis, such as blood brain barrier leakage that can be induced by...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
08/13/2021
Application: IHC-Fr
Species: Human

Verified Customer
11/04/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol L1CAM