L1CAM Antibody (UJ127.11)

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IP, CyTOF-ready, ICC/IF
Clone
UJ127.11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

L1CAM Antibody (UJ127.11) Summary

Immunogen
Homogenous suspension of 16 week human foetal brain.
Localization
Cell Membrane
Marker
Axon Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
L1CAM
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 0.5 - 1.0 ug/10^6 cells
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
  • Immunofluorescence 1-2 ug/ml
Application Notes
This antibody is Cytof ready.
Control
L1CAM Knockout HeLa Cell Lysate
Publications
Read Publication using
NB100-2682 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
IgG purified

Notes

Positive control(s): Frozen mouse E16.5 lung sections (IHC), Cell lines TR 14, LAN-1, CHP 100, CHP212, CHP126 ( Neuroblastomas or Schwannomas) (IF), M5 melanoma cells or human foetal brain (WB).

Alternate Names for L1CAM Antibody (UJ127.11)

  • antigen identified by monoclonal R1
  • CAML1
  • CAML1N-CAML1
  • CD171 antigen
  • CD171
  • HSAS
  • HSAS1
  • L1 cell adhesion molecule
  • L1CAM
  • MASA
  • MIC5
  • N-CAM-L1
  • NCAM-L1
  • neural cell adhesion molecule L1
  • S10
  • SPG1

Background

The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for L1CAM Antibody (NB100-2682)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
All Applications
Filter By Species
Human
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for L1CAM Antibody (NB100-2682) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2682AF350
Alexa Fluor 405 NB100-2682AF405
Alexa Fluor 488 NB100-2682AF488
Alexa Fluor 532 NB100-2682AF532
Alexa Fluor 594 NB100-2682AF594
Alexa Fluor 647 NB100-2682AF647
Alexa Fluor 700 NB100-2682AF700
Alexa Fluor 750 NB100-2682AF750
Allophycocyanin/Cy7 NB100-2682APCCY7
Biotin NB100-2682B
DyLight 350 NB100-2682UV
DyLight 405 NB100-2682V
DyLight 488 NB100-2682G
DyLight 550 NB100-2682R
DyLight 594 NB100-2682DL594
DyLight 650 NB100-2682C
DyLight 680 NB100-2682FR
DyLight 755 NB100-2682IR
FITC NB100-2682F
HRP NB100-2682H
Janelia Fluor 549 NB100-2682JF549
Janelia Fluor 646 NB100-2682JF646
PE/Atto594 NB100-2682PEATT594
PE/Cy5.5 NB100-2682PECY55
PE/Cy7 NB100-2682PECY7
PerCP NB100-2682PCP

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Bioinformatics Tool for L1CAM Antibody (NB100-2682)

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Blogs on L1CAM.

Identifying tumoral and stromal transcriptomes that underlie tumor plasticity and stromal neuroinflammatory response in brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Cancers in the brain often come from tumors elsewhere in the body. Several adaptive mechanisms influence brain metastasis, such as blood brain barrier leakage that can be induced by...  Read full blog post.

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Bioinformatics

Gene Symbol L1CAM