SIN3A Antibody (8V5B6)

Western Blot: SIN3A Antibody (8V5B6) [NBP3-16124] - Western blot analysis of extracts of various cell lines, using SIN3A antibody (NBP3-16124) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody (8V5B6) [NBP3-16124] - Confocal imaging of NIH/3T3 cells using SIN3A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded human small intestine tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded rat testis tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded mouse brain tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded rat colon tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded rat spleen tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded mouse colon tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded mouse testis tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody (8V5B6) [NBP3-16124] - Confocal imaging of U-2 OS cells using SIN3A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded human esophagus tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 8V5B6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SIN3A Antibody (8V5B6) Summary

• Description: Novus Biologicals Rabbit SIN3A Antibody (8V5B6) (NBP3-16124) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1174-1273 of human SIN3A (Q96ST3). YKMVYVIKSEDYMYRRTALLRAHQSHERVSKRLHQRFQAWVDKWTKEHVPREMAAETSKWLMGEGLEGLVPCTTTCDTETLHFVSINKYRVKYGTVFKAP
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SIN3A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SIN3A Antibody (8V5B6)

• DKFZp434K2235
• FLJ90319
• Histone deacetylase complex subunit Sin3a
• KIAA0700
• paired amphipathic helix protein Sin3a
• SIN3 homolog A, transcription regulator (yeast)
• SIN3 homolog A, transcriptional regulator (yeast)
• SIN3A
• Transcriptional corepressor Sin3a
• transcriptional co-repressor Sin3A
• transcriptional regulator, SIN3A

Background

Sin3A is encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIN3A