SIGNR3/CD209d Antibody

Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse SIGNR3/CD209d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SIGNR3/CD209d at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SIGNR3/CD209d Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SIGNR3/CD209d
  Glu82-Lys237
  Accession # Q91ZW8
• Specificity: Detects mouse SIGNR3/CD209d in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) DC-SIGN R, rhDC-SIGN, recombinant mouse (rm) SIGN R1, rmSIGN R4, and rmSIGN R7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Cd209d
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIGNR3/CD209d Antibody

• CD209 antigen;CDSIGN;CLEC4L;DC-SIGN;DC-SIGN1;hDC-SIGN
• CD209d
• SIGNR3

Background

SIGNR3 (Specific Intercellular adhesion molecule-3-Grabbing Non-integrin Related gene 3; also CD209d and DC-SIGNR3) is a 30-34 kDa member of the C-type lectin family of proteins. It is found on macrophages, DC and monocytes, and appears to bind both high mannose and fucose moieties on the surface of extracellular pathogens. It is particularly important in clearing Mycobacterium tuberculosis. Upon ligation, SIGNR3 is internalized and presumably enters the endosomal system. Notably, and unlike in human, there are multiple SIGNs in mouse, and SIGNR3 most closely resembles the biology of human DC-SIGN. Mouse SIGNR3 is a 237 amino acid (aa) type II transmembrane glycoprotein. It contains a 54 aa N-terminal cytoplasmic region plus a 162 aa C-terminal extracellular domain (aa 76-237) that possesses a C-type lectin domain (aa 106-228). It is suggested that mouse SIGNR3 may oligomerize. There are two potential splice variants. One possesses an alternative start site 23 aa upstream of the standard site, while a second shows a deletion of aa 45-74. Over aa 82-237, mouse SIGNR3 shares 89% and 59% aa sequence identity with rat and human SIGNR3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIGNR3/CD209d Antibody (AF7220)(1)

Publication using AF7220

• KJ Rogers, B Brunton, L Mallinger, D Bohan, KM Sevcik, J Chen, N Ruggio, W Maury IL-4/IL-13 polarization of macrophages enhances Ebola virus glycoprotein-dependent infection PLoS Negl Trop Dis, 2019-12-11;13(12):e0007819. 2019-12-11 [PMID: 31825972] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Cd209d
• Uniprot:
  • Q91ZW8(Mouse)