SIGNR3/CD209d Antibody [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

SIGNR3/CD209d Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SIGNR3/CD209d
  Glu82-Lys237
  Accession # Q91ZW8
• Specificity: Detects mouse SIGNR3/CD209d in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) DC-SIGN R, rhDC-SIGN, recombinant mouse (rm) SIGN R1, rmSIGN R4, and rmSIGN R7 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIGNR3/CD209d Antibody [Alexa Fluor® 647]

• CD209 antigen;CDSIGN;CLEC4L;DC-SIGN;DC-SIGN1;hDC-SIGN
• CD209d
• SIGNR3

Background

SIGNR3 (Specific Intercellular adhesion molecule-3-Grabbing Non-integrin Related gene 3; also CD209d and DC-SIGNR3) is a 30-34 kDa member of the C-type lectin family of proteins. It is found on macrophages, DC and monocytes, and appears to bind both high mannose and fucose moieties on the surface of extracellular pathogens. It is particularly important in clearing Mycobacterium tuberculosis. Upon ligation, SIGNR3 is internalized and presumably enters the endosomal system. Notably, and unlike in human, there are multiple SIGNs in mouse, and SIGNR3 most closely resembles the biology of human DC-SIGN. Mouse SIGNR3 is a 237 amino acid (aa) type II transmembrane glycoprotein. It contains a 54 aa N-terminal cytoplasmic region plus a 162 aa C-terminal extracellular domain (aa 76-237) that possesses a C-type lectin domain (aa 106-228). It is suggested that mouse SIGNR3 may oligomerize. There are two potential splice variants. One possesses an alternative start site 23 aa upstream of the standard site, while a second shows a deletion of aa 45-74. Over aa 82-237, mouse SIGNR3 shares 89% and 59% aa sequence identity with rat and human SIGNR3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.