Sigma-1 R/OPRS1 Antibody - BSA Free

Western Blot: Sigma-1 R/OPRS1 Antibody [NBP2-19628] - Wild-type (WT) and SIGMAR1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Sigma-1 R Antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Sigma-1 R/OPRS1 Antibody [NBP2-19628] - Analysis of paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.

Western Blot: Sigma-1 R/OPRS1 Antibody [NBP2-19628] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Sigma-1 R/OPRS1 antibody [N1C3] (NBP2-19628) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Sigma-1 R/OPRS1 Antibody [NBP2-19628] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Sigma-1 R/OPRS1 antibody [N1C3] (NBP2-19628) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Sigma-1 R/OPRS1 Antibody [NBP2-19628] - Wild-type (WT) andSIGMAR1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Ze, Mu, Rt, Bv, Gp, RM
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Sigma-1 R/OPRS1 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit Sigma-1 R/OPRS1 Antibody - BSA Free (NBP2-19628) is a polyclonal antibody validated for use in IHC and WB. Anti-Sigma-1 R/OPRS1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Sigma-1 R/OPRS1. The exact sequence is proprietary.
• Localization: Nucleus inner membrane; Nucleus outer membrane; Endoplasmic reticulum membrane; Lipid droplet; Cell junction; Cell membrane; Cell projection; growth cone
• Predicted Species: Mouse (94%), Rat (96%), Rhesus Macaque (100%), Bovine (97%), Guinea Pig (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIGMAR1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Sigma-1 R/OPRS1 Antibody - BSA Free

• Aging-associated gene 8 protein
• ALS16
• FLJ25585
• hSigmaR1
• OPRS1
• SIG-1R
• sigma 1
• Sigma 1-type opioid receptor
• sigma non-opioid intracellular receptor 1
• Sigma-1 R
• sigma1 receptor
• Sigma1R
• Sigma-1R
• sigma1-receptor
• SIGMAR1
• SR31747 binding protein 1
• SR31747-binding protein
• SRBP
• SR-BP
• SR-BP1
• SRBPMGC3851
• type I sigma receptor

Background

This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Alternative splicing of this gene results in transcript variants encoding distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Sigma-1 R/OPRS1 Antibody (NBP2-19628)(1)

Publication using NBP2-19628

• Lopez OV, Gorantla S, Segarra AC et al. Sigma-1 Receptor Antagonist (BD1047) Decreases Cathepsin B Secretion in HIV-Infected Macrophages Exposed to Cocaine. J Neuroimmune Pharmacol. 2018-10-10 [PMID: 30306495] (IHC-P, Human)

Bioinformatics

• Gene Symbol: SIGMAR1
• Uniprot:
  • Q99720()