Siglec-H Antibody (23M15C8) - BSA Free

Immunohistochemistry-Paraffin: Siglec-H Antibody (23M15C8) [NBP2-27062] - Analysis of Siglec-H in mouse spleen tissue using Siglec-H antibody

Product Discontinued

Siglec-H Antibody (23M15C8) - BSA Free Summary

• Immunogen: A portion of amino acids 50-100 of mouse Siglec-H was used as the immunogen for this antibody.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: Siglech
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Siglec-H Antibody (23M15C8) - BSA Free

• 6430529G09Rik
• Siglech sialic acid binding Ig-like lectin H
• SiglecH
• Siglec-H

Background

Siglec-H, or sialic acid binding immunoglobulin-like lectin H, is a CD33 related protein expressed specifically by plasmacytoid dendritic cells or pDCs (1, 2). Antigen-mediated delivery by Siglec-H in pDCs inhibits Th cell proliferation and further antibody responsesThis leads to lessened expansion and Th1/Th17 polarization (3). Constant and low density antigen presentation by Siglec-H is thought to lead to an exhaustive type lessening of the response in CD4+ cells but not tolerance. A number of pathways have been proposed for the Siglec-H induced T cell hyporesponsiveness. Ever decreasing activation cycles in the presence of low level but continuous antigen delivery, as that observed with Siglec-H, have also been demonstrated to impart FoxP3+ Tregs immunosuppressive tolerogen-like effects (4, 5). Ability to identify and control Siglec-H antigen mediated delivery activities with specific antibody provides a focal point for potential development of inflammation controls.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-H Antibody (NBP2-27062)(2)

Publications using NBP2-27062

• Rosales IA, Yang C, Farkash EA Et al. Novel intragraft regulatory lymphoid structures in kidney allograft tolerance American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2021-11-02 [PMID: 34726836]
• Scholz T, Weigert A, Brune B et al. GM-CSF in murine psoriasiform dermatitis: Redundant and pathogenic roles uncovered by antibody-induced neutralization and genetic deficiency PLoS ONE 2017-08-04 [PMID: 28777803] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: Siglech
• Uniprot:
  • Q3Y597(Human)