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Siglec-H Antibody (23M15C8)

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Immunohistochemistry-Paraffin: Siglec-H Antibody (23M15C8) [NBP2-27062] - Analysis of Siglec-H in mouse spleen tissue using Siglec-H antibody
Immunohistochemistry-Paraffin: Siglec-H Antibody (23M15C8) [NBP2-27062] - Analysis of Siglec-H in mouse spleen tissue using Siglec-H antibody

Product Details

Summary
Reactivity MuSpecies Glossary
Applications IHC, IHC-P
Clone
23M15C8
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-27062AF350
Alexa Fluor 405
NBP2-27062AF405
Alexa Fluor 488
NBP2-27062AF488
Alexa Fluor 532
NBP2-27062AF532
Alexa Fluor 594
NBP2-27062AF594
Alexa Fluor 647
NBP2-27062AF647
Alexa Fluor 700
NBP2-27062AF700
Alexa Fluor 750
NBP2-27062AF750
Biotin
NBP2-27062B
DyLight 350
NBP2-27062UV
DyLight 405
NBP2-27062V
DyLight 488
NBP2-27062G
DyLight 550
NBP2-27062R
DyLight 650
NBP2-27062C
DyLight 680
NBP2-27062FR
DyLight 755
NBP2-27062IR
HRP
NBP2-27062H
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Siglec-H Antibody (23M15C8) Summary

Immunogen
A portion of amino acids 50-100 of mouse Siglec-H was used as the immunogen for this antibody.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
Siglech
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
Publications
Read Publication using
NBP2-27062 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Siglec-H antibody at 0.5 mg/ml in PBS containing 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Siglec-H Antibody (23M15C8)

  • 6430529G09Rik
  • Siglech sialic acid binding Ig-like lectin H
  • SiglecH
  • Siglec-H

Background

Siglec-H, or sialic acid binding immunoglobulin-like lectin H, is a CD33 related protein expressed specifically by plasmacytoid dendritic cells or pDCs (1, 2). Antigen-mediated delivery by Siglec-H in pDCs inhibits Th cell proliferation and further antibody responsesThis leads to lessened expansion and Th1/Th17 polarization (3). Constant and low density antigen presentation by Siglec-H is thought to lead to an exhaustive type lessening of the response in CD4+ cells but not tolerance. A number of pathways have been proposed for the Siglec-H induced T cell hyporesponsiveness. Ever decreasing activation cycles in the presence of low level but continuous antigen delivery, as that observed with Siglec-H, have also been demonstrated to impart FoxP3+ Tregs immunosuppressive tolerogen-like effects (4, 5). Ability to identify and control Siglec-H antigen mediated delivery activities with specific antibody provides a focal point for potential development of inflammation controls.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody.
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Knockdown Validated: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY licence.
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(CB19)
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Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on Ramos cells with CD19 Antibody (CB19) NBP2-26646 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test.
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Siglec-H Antibody
(23M15C8)
NBP2-27062
Siglec-H Antibody (23M15C8)
Species: Mu
Applications: IHC, IHC-P
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Publications for Siglec-H Antibody (NBP2-27062)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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Secondary Antibodies

 

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Other Available Formats

Alexa Fluor 350 NBP2-27062AF350
Alexa Fluor 405 NBP2-27062AF405
Alexa Fluor 488 NBP2-27062AF488
Alexa Fluor 532 NBP2-27062AF532
Alexa Fluor 594 NBP2-27062AF594
Alexa Fluor 647 NBP2-27062AF647
Alexa Fluor 700 NBP2-27062AF700
Alexa Fluor 750 NBP2-27062AF750
Biotin NBP2-27062B
DyLight 350 NBP2-27062UV
DyLight 405 NBP2-27062V
DyLight 488 NBP2-27062G
DyLight 550 NBP2-27062R
DyLight 650 NBP2-27062C
DyLight 680 NBP2-27062FR
DyLight 755 NBP2-27062IR
HRP NBP2-27062H
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Bioinformatics

Gene Symbol Siglech
Uniprot