Siglec-9 Antibody

Western Blot: Siglec-9 Antibody [NBP2-27070] - Analysis using Siglec-9 antibody. Lysate from human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat liver. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Bv, Ca, Eq, Pm, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Siglec-9 Antibody Summary

• Immunogen: A portion of amino acids 40-90 of human Siglec9 was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIGLEC9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-4ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in dwarf chimpanzee.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for Siglec-9 Antibody

• CD329
• CDw329
• FOAP-9
• OBBP-Like
• Protein FOAP-9
• sialic acid binding Ig-like lectin 9
• sialic acid-binding Ig-like lectin 9
• Siglec9
• Siglec-9

Background

Siglec9 (Sialic acid-binding Ig-like lectin 9) is single-pass type I membrane protein and belongs to immunoglobulin super family and contains two Ig-like C2 type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. It is reported that Siglec9 recognizes sialic acid in alpha2, 3-or alpha2, 6-glycosidic linkage to galactose. Using a specific antibody, Siglec9 was found to be expressed at high or intermediate levels by monocytes, neutrophils, and a minor population of CD16+, CD56- cells. Siglec9 also acts as an inhibitory receptor for MUC16 that attenuates T- and Nk cell function. Recent reports suggest that Siglec9 expressed on DCs is involved in immuno regulation through ligation with mucins in an epithelial cancer patient.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIGLEC9
• Uniprot:
  • Q9Y336()
  • Q9Y336()