LAIR1 Antibody (NKTA255) - BSA Free

Flow (Cell Surface): LAIR1 Antibody (NKTA255) [NBP2-62214] - Surface staining of CD305 / LAIR-1 in human peripheral blood with anti-CD305 (NKTA255) PerCP-CyTM5.5.

Flow (Cell Surface): LAIR1 Antibody (NKTA255) [NBP2-62214] - Surface staining of CD305 / LAIR-1 in human peripheral blood (lymphocyte gate) with anti-CD305 (NKTA255) PE.

Surface staining of human lymphocytes stained using anti-human CD305 (NKTA255) purified antibody (concentration in sample 2 ug/ml, GAM APC) and anti-human CD19 (LT19) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

• Reactivity: Hu
• Applications: WB, Flow, IP
• Clone: NKTA255
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

LAIR1 Antibody (NKTA255) - BSA Free Summary

• Description: Novus Biologicals Mouse LAIR1 Antibody (NKTA255) - BSA Free (NBP2-62214) is a monoclonal antibody validated for use in WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Clone NKTA255 was used by HLDA to establish CD designation.
• Immunogen: Activated NK cells and CD3- thymocytes
• Specificity: The mouse monoclonal antibody NKTA255 recognizes CD305 / LAIR1, a 40 kDa type I transmembrane glycoprotein expressed on NK, T, and B cells, monocytes, dendritic cells, eosinophils, basophils, mast cells, CD34+ hematopoietic progenitor cells and thymocytes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAIR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-4 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Purity: Protein A purified

Alternate Names for LAIR1 Antibody (NKTA255) - BSA Free

• CD305 antigen
• CD305
• CD305leukocyte-associated Ig-like receptor 1
• HLAIR1
• LAIR1
• LAIR-1
• leukocyte-associated immunoglobulin-like receptor 1

Background

LAIR1 is a transmembrane glycoprotein(approx. 32kDa) expressed on the majority of human peripheral blood mononuclear leukocytes including T cells, B cells, NK cells, macrophages and dendritic cells. LAIR1 recruits SHP1 and SHP2 tyrosine phosphatases upon activation, and cross-linking of the LAIR1 molecule on NK cells results in strong inhibition of NK cell mediated cytotoxicity. This clone is reported to inhibit NK cell triggering via CD16 molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for LAIR1 Antibody (NBP2-62214) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/15/2021

Summary

• Application: Western Blot
• Sample Tested: Dendritic cells
• Species: Human
• Lot: NKTA255

Comments

• Comments: Primary antibody dilution 1:1000

Bioinformatics

• Gene Symbol: LAIR1