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FAB1137P) and (B) Mouse Anti-Human Siglec-9 Monoclonal Antibody (Catalog # MAB1139, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PerCP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0114)." class="big_lightbox" target="_blank">
1139-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-9 Fc Chimera (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec-9 Monoclonal Antibody (Catalog # MAB1139). The ND50 is typically 0.015-0.075 µg/mL." class="big_lightbox" target="_blank">
1139-SL) was serially diluted and captured by Mouse Anti-Human Siglec‑9 Monoclonal Antibody (Catalog # MAB1139) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Siglec‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1139) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
1139-SL) was serially diluted and captured by Mouse Anti-Human Siglec‑9 Monoclonal Antibody (Catalog # MAB1139) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Siglec‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1139) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface