Siglec-10 Antibody

Human whole blood CD19+ B cells were stained with Goat Anti-Human Siglec‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2130, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phy­coerythrin-conjugated Anti-Goat IgG Secondary Anti­body (Catalog # F0107).

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Siglec-10 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Siglec-10
  Met17-Thr546
  Accession # Q96LC7
• Specificity: Detects human Siglec‑10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human (rh) Siglec-5 is observed and less than 1% cross-reactivity with rhSiglec-2, rhSiglec-3, rhSiglec-7, rhSiglec-9, and recombinant mouse Siglec-F is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIGLEC10
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-10 Antibody

• MGC126774
• PRO940
• sialic acid binding Ig-like lectin 10
• sialic acid-binding Ig-like lectin 10
• Siglec10
• Siglec-10
• siglec-like gene 2
• Siglec-like protein 2
• SLG2
• SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5‑11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1‑3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V‑type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5‑8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5‑7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is up‑regulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (7). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (5, 9).

1. Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
2. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
3. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
4. Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
5. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
6. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900.
7. Li, N. et al. (2001) J. Biol. Chem. 276:28106.
8. Munday, J. et al. (2001) Biochem. J. 355:489.
9. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
10. Aizawa, H. et al. (2003) Genomics 82:521.
11. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-10 Antibody (AF2130)(3)

Publications using AF2130

• M Vijayan, CL Lee, VHH Wong, X Wang, K Bai, J Wu, H Koistinen, M Seppälä, KF Lee, WSB Yeung, EHY Ng, PCN Chiu Decidual glycodelin-A polarizes human monocytes towards a decidual macrophage-like phenotype via siglec-7 J. Cell. Sci., 2020-07-23;0(0):. 2020-07-23 [PMID: 32513821] (Neutralization, Human)
• J Xie, I Christiaen, B Yang, I Trus, B Devriendt, T Cui, R Wei, HJ Nauwynck Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells Vet. Res., 2018-07-18;49(1):67. 2018-07-18 [PMID: 30021620] (ICC, Porcine)
• Yokoi H, Myers A, Matsumoto K, Crocker PR, Saito H, Bochner BS Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells. Allergy, 2006-06-01;61(6):769-76. 2006-06-01 [PMID: 16677248] (Western Blot, Human)

Review for Siglec-10 Antibody (AF2130) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Immunofluorescence - fresh-frozen Human 02/29/2020

Summary

• Application: Other
• Sample Tested: Colon tissue
• Species: Human
• Lot: N/A

Bioinformatics

• Gene Symbol: SIGLEC10
• Uniprot:
  • Q96LC7()