Siglec-10 Antibody

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Human whole blood CD19+ B cells were stained with Goat Anti-Human Siglec‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2130, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Siglec-10 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-10
Met17-Thr546
Accession # Q96LC7
Specificity
Detects human Siglec‑10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human (rh) Siglec-5 is observed and less than 1% cross-reactivity with rhSiglec-2, rhSiglec-3, rhSiglec-7, rhSiglec-9, and recombinant mouse Siglec-F is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SIGLEC10
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 4
using
AF2130 in the following applications:

Publications
Read Publications using
AF2130 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-10 Antibody

  • MGC126774
  • PRO940
  • sialic acid binding Ig-like lectin 10
  • sialic acid-binding Ig-like lectin 10
  • Siglec10
  • Siglec-10
  • siglec-like gene 2
  • Siglec-like protein 2
  • SLG2
  • SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5‑11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1‑3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V‑type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5‑8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5‑7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is up‑regulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (7). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (5, 9).

  1. Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
  2. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  3. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
  4. Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
  5. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
  6. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900.
  7. Li, N. et al. (2001) J. Biol. Chem. 276:28106.
  8. Munday, J. et al. (2001) Biochem. J. 355:489.
  9. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
  10. Aizawa, H. et al. (2003) Genomics 82:521.
  11. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-10 Antibody (AF2130)(2)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 2 applications: ICC, Western Blot.


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Western Blot
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Review for Siglec-10 Antibody (AF2130) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2130:
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Images Ratings Applications Species Date Details
 Siglec-10 AF2130
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reviewed by:
Anonymous
Human 02/29/2020
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Summary

Sample TestedColon tissue
SpeciesHuman
LotN/A

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Biotin BAF2130

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Recent Reviews

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Anonymous
02/29/2020
Application:
Species: Human

Bioinformatics

Gene Symbol SIGLEC10
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