Siglec-8 Antibody (837535) [Unconjugated]

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Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 PE-conjugated Monoclonal Antibody (Catalog # FAB155P) and either (A) Mouse Anti-Human Siglec-8 Monoclonal Antibody (Catalog # MAB7975) or (B) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
837535
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Siglec-8 Antibody (837535) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-8
Met17-Ala363
Accession # Q9NYZ4
Specificity
Detects human Siglec-8 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Siglec-7, -9, or -14 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC8
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 4
using
MAB7975 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-8 Antibody (837535) [Unconjugated]

  • CD329 antigen
  • CDw329
  • MGC59785
  • SAF2
  • SAF2SAF-2
  • sialic acid binding Ig-like lectin 8
  • Sialoadhesin family member 2
  • Siglec8
  • Siglec-8
  • SIGLEC8L
  • SIGLEC8Lsialic acid-binding Ig-like lectin 8

Background

Siglec-8 (Sialic acid-binding Ig-like lectin 8), formerly called SAF-2 (sialoadhesin family member 2) and designated CD329, is a 75 kDa member of the inhibitory
CD33‑related SIGLEC family within the Ig superfamily of proteins. An alternately spliced 48 kDa short form lacks most of the intracellular domain, while another isoform lacks the second of three extracellular Ig domains, representing amino acids (aa) 152-245 of the 499 aa long form. Siglec-8 is expressed exclusively on eosinophils, mast cells, and weakly on basophils, and is a marker for eosinophil terminal differentiation. It specifically recognizes 6’ sulfated sialyl Lewis X carbohydrate structures. Engagement results in eosinophils apoptosis, or mast cell inhibition of degranulation. Eosinophil Siglec F is the mouse paralog of human Siglec-8, but shares <40% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-8 Antibody (MAB7975) (0)

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Review for Siglec-8 Antibody (MAB7975) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB7975:
Filter by Applications
IHC-Fr
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Siglec-8 MAB7975
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reviewed by:
Anonymous
IHC-Fr Human 03/30/2020
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedTonsil
SpeciesHuman
LotN/A

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Recent Reviews

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2
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1
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Anonymous
03/30/2020
Application: IHC-Fr
Species: Human

Bioinformatics

Gene Symbol SIGLEC8
Uniprot