Siglec-8 Antibody (837535) [Phycoerythrin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
837535
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Siglec-8 Antibody (837535) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-8
Met17-Ala363
Accession # Q9NYZ4
Specificity
Detects human Siglec-8 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Siglec-7, -9, or -14 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC8
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publication using
FAB7975P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-8 Antibody (837535) [Phycoerythrin]

  • CD329 antigen
  • CDw329
  • MGC59785
  • SAF2
  • SAF2SAF-2
  • sialic acid binding Ig-like lectin 8
  • Sialoadhesin family member 2
  • Siglec8
  • Siglec-8
  • SIGLEC8L
  • SIGLEC8Lsialic acid-binding Ig-like lectin 8

Background

Siglec-8 (Sialic acid-binding Ig-like lectin 8), formerly called SAF-2 (sialoadhesin family member 2) and designated CD329, is a 75 kDa member of the inhibitory CD33‑related SIGLEC family within the Ig superfamily of proteins. An alternately spliced 48 kDa short form lacks most of the intracellular domain, while another isoform lacks the second of three extracellular Ig domains, representing amino acids (aa) 152-245 of the 499 aa long form. Siglec-8 is expressed exclusively on eosinophils, mast cells, and weakly on basophils, and is a marker for eosinophil terminal differentiation. It specifically recognizes 6’ sulfated sialyl Lewis X carbohydrate structures. Engagement results in eosinophils apoptosis, or mast cell inhibition of degranulation. Eosinophil Siglec F is the mouse paralog of human Siglec-8, but shares <40% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-8 Antibody (FAB7975P)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol SIGLEC8
Uniprot