Siglec-8 Antibody (837535) [Allophycocyanin]

Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 PE-conjugated Monoclonal Antibody (Catalog # FAB155P) and either (A) Mouse Anti-Human Siglec-8 APC-conjugated Monoclonal Antibody (Catalog # FAB7975A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 837535
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Siglec-8 Antibody (837535) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Siglec-8
  Met17-Ala363
  Accession # Q9NYZ4
• Specificity: Detects human Siglec-8 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Siglec-7, -9, or -14 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIGLEC8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-8 Antibody (837535) [Allophycocyanin]

• CD329 antigen
• CDw329
• MGC59785
• SAF2
• SAF2SAF-2
• sialic acid binding Ig-like lectin 8
• Sialoadhesin family member 2
• Siglec8
• Siglec-8
• SIGLEC8L
• SIGLEC8Lsialic acid-binding Ig-like lectin 8

Background

Siglec-8 (Sialic acid-binding Ig-like lectin 8), formerly called SAF-2 (sialoadhesin family member 2) and designated CD329, is a 75 kDa member of the inhibitory CD33‑related SIGLEC family within the Ig superfamily of proteins. An alternately spliced 48 kDa short form lacks most of the intracellular domain, while another isoform lacks the second of three extracellular Ig domains, representing amino acids (aa) 152-245 of the 499 aa long form. Siglec-8 is expressed exclusively on eosinophils, mast cells, and weakly on basophils, and is a marker for eosinophil terminal differentiation. It specifically recognizes 6’ sulfated sialyl Lewis X carbohydrate structures. Engagement results in eosinophils apoptosis, or mast cell inhibition of degranulation. Eosinophil Siglec F is the mouse paralog of human Siglec-8, but shares <40% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIGLEC8
• Uniprot:
  • Q9NYZ4()