Siglec-9 Antibody (191240) [Unconjugated]

Images

 
Recombinant Human Siglec‑9 Fc Chimera (Catalog # 1139-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human ...read more
Human peripheral blood granulocytes werestained with (A) Mouse Anti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody(Catalog # FAB1137P) and (B) MouseAnti-HumanSiglec‑9 Monoclonal Antibody (Catalog # MAB1139, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, ELISA(Cap)
Clone
191240
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Siglec-9 Antibody (191240) [Unconjugated] Summary

Additional Information
Clone 191240 was used by HLDA to establish CD designation
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-9
Gln18-Gly348 (predicted)
Accession # Q9Y336
Specificity
Detects human Siglec-9 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse
Siglec‑E is observed and less than 1% cross-reactivity with recombinant human (rh) Siglec-3, rhSiglec-5, rhSiglec-6, rhSiglec-7, rhSiglec-8 or rhSiglec-10 is observed. In Western blots, approximately 100% cross-reactivity with recombinant mouse Siglec‑E is observed under
non-reduced conditions.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC9
Endotoxin Note
<1.0 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.015-0.075 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human Siglec-9 Antibody (Catalog # MAB1139)
ELISA Detection: Human Siglec-9 Biotinylated Antibody (Catalog # BAF1139)
Standard: Recombinant Human Siglec-9 Fc Chimera (Catalog # 1139-SL)
Publications
Read Publications using
MAB1139 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-9 Antibody (191240) [Unconjugated]

  • CD329
  • CDw329
  • FOAP-9
  • OBBP-Like
  • Protein FOAP-9
  • sialic acid binding Ig-like lectin 9
  • sialic acid-binding Ig-like lectin 9
  • Siglec9
  • Siglec-9

Background

Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1‑4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.

  1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
  2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  3. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  5. Zhang, J.Q. et al. (2000) J. Biol. Chem. 275:22121.
  6. Angata, T. et al. (2000) J. Biol. Chem. 275:22127.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-9 Antibody (MAB1139)(3)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1139U
Alexa Fluor 405 FAB1139V
Alexa Fluor 488 FAB1139G
Alexa Fluor 488 FAB1139G
Alexa Fluor 594 FAB1139T
Alexa Fluor 647 FAB1139R
Alexa Fluor 700 FAB1139N
Alexa Fluor 700 FAB1139N
Alexa Fluor 750 FAB1139S
Allophycocyanin FAB1139A
Allophycocyanin FAB1139A
Fluorescein FAB1139F
Fluorescein FAB1139F
Phycoerythrin FAB1139P
Phycoerythrin FAB1139P

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Bioinformatics

Gene Symbol SIGLEC9
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