SIAH1/2 Antibody (8G7H12)

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Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596] - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA ...read more

Product Details

Summary
Reactivity Hu, Rt, Po, Dr, Ze, Mu(-)Species Glossary
Applications WB, IP
Clone
8G7H12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SIAH1/2 Antibody (8G7H12) Summary

Immunogen
Synthetic peptide made to a C-terminal portion of the drosophila SINA protein (within residues 280-331). [Swiss-Prot# P21461]
Localization
Nuclear, Cytoplasmic
Specificity
This is specific for human SIAH 1 and 2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIAH1
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This SIAH1/2 antibody is useful for Immunoprecipitation and Western blot, where a band is seen at ~37 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB110-93596 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human, porcine, rat, zebrafish and Drosophila. Does not react with mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tissue culture supernatant
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for SIAH1/2 Antibody (8G7H12)

  • E3 ubiquitin-protein ligase SIAH1
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ08065
  • hSIAH1
  • HUMSIAH
  • seven in absentia homolog 1 (Drosophila)
  • Seven in absentia homolog 1
  • Siah-1
  • SIAH1A
  • siah-1a

Background

The Drosophila SINA (seven in absentia) protein is an E3 ubiquitin ligase component of the RAS signal transduction pathway. The RAS signal pathway controls cell proliferation, differentiation, and survival, and regulation of this pathway is critical for normal development. In Drosophila SINA serves as a downstream gatekeeper required for proper RAS signal transduction. Similarly to SINA in Drosophila, the human protein SIAH has also been shown to be required for oncogenic RAS signaling in cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SIAH1/2 Antibody (NB110-93596)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol SIAH1
Entrez
Uniprot