SIAH1/2 Antibody (8G7H12) - BSA Free

Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596] - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly speci

• Reactivity: Hu, Rt, Po, Dr, Ze, Mu(-)
• Applications: WB, IP
• Clone: 8G7H12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

SIAH1/2 Antibody (8G7H12) - BSA Free Summary

• Immunogen: Synthetic peptide made to a C-terminal portion of the drosophila SINA protein (within residues 280-331). [Swiss-Prot# P21461]
• Localization: Nuclear, Cytoplasmic
• Specificity: This is specific for human SIAH 1 and 2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIAH1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: This SIAH1/2 antibody is useful for Immunoprecipitation and Western blot, where a band is seen at ~37 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: 0.1% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for SIAH1/2 Antibody (8G7H12) - BSA Free

• E3 ubiquitin-protein ligase SIAH1
• EC 6.3.2
• EC 6.3.2.-
• FLJ08065
• hSIAH1
• HUMSIAH
• seven in absentia homolog 1 (Drosophila)
• Seven in absentia homolog 1
• Siah-1
• SIAH1A
• siah-1a

Background

The Drosophila SINA (seven in absentia) protein is an E3 ubiquitin ligase component of the RAS signal transduction pathway. The RAS signal pathway controls cell proliferation, differentiation, and survival, and regulation of this pathway is critical for normal development. In Drosophila SINA serves as a downstream gatekeeper required for proper RAS signal transduction. Similarly to SINA in Drosophila, the human protein SIAH has also been shown to be required for oncogenic RAS signaling in cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIAH1/2 Antibody (NB110-93596)(1)

Publication using NB110-93596

• Schmidt, RL et al. Inhibition of RAS-mediated transformation and tumorigenesis by targeting the downstream E3 ubiquitin ligase seven in absentia homologue. Cancer Res;67(24):11798-810. 2007-12-15 [PMID: 18089810] (WB, Human)

Bioinformatics

• Gene Symbol: SIAH1
• Entrez:
  • 6477(Human)
  • 6478(Human)
• Uniprot:
  • NP_005058.2(Human)