SIAH2 Antibody (24E6H3)


Immunocytochemistry/ Immunofluorescence: SIAH2 Antibody (24E6H3) [NB110-88113] - Immunofluorescent staining of SIAH in ovarian carcinoma (OVCR3)cells using NB110-88113.
Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Staining of SIAH in human colorectal adenocarcinoma using NB110-88113. Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. more

Product Details

Reactivity Hu, Po, Dr, Mu(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

SIAH2 Antibody (24E6H3) Summary

Synthetic peptide made to an N-terminal portion of the drosophila SINA protein (within residues 1-100). [Swiss-Prot# P21461]
Nuclear, Cytoplasmic
This is specific for human SIAH2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.1% Sodium Azide
Tissue culture supernatant


  • Western Blot
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:10
  • Immunohistochemistry 1:40-1:50
  • Immunohistochemistry-Frozen 1:40-1:50
  • Immunohistochemistry-Paraffin 1:40-1:50
Application Notes
This SIAH2 (24E6H3) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections.
Read Publications using
NB110-88113 in the following applications:

Reactivity Notes

Human, Porcine, Drosophila. Does not react with mouse.

Alternate Names for SIAH2 Antibody (24E6H3)

  • E3 ubiquitin-protein ligase SIAH2
  • EC 6.3.2
  • EC 6.3.2.-
  • hSiah2
  • seven in absentia (Drosophila) homolog 2
  • seven in absentia homolog 2 (Drosophila)
  • Seven in absentia homolog 2
  • siah-2


The Drosophila SINA (seven in absentia) protein is an E3 ubiquitin ligase component of the RAS signal transduction pathway. The RAS signal pathway controls cell proliferation, differentiation, and survival, and regulation of this pathway is critical for normal development. In Drosophila SINA serves as a downstream gatekeeper required for proper RAS signal transduction. Similarly to SINA in Drosophila, the human protein SIAH has also been shown to be required for oncogenic RAS signaling in cancer.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SIAH2 Antibody (NB110-88113)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB110-88113 Applications Species
van der Willik KD, Timmermans MM. SIAH2 protein expression in breast cancer is inversely related with ER status and outcome to tamoxifen therapy. Am J Cancer Res. Feb 1 2016 12:00AM [PMID:27186402] (IHC-P, Human) IHC-P Human
Ma B, Cheng H, Gao R et al. Zyxin-Siah2-Lats2 axis mediates cooperation between Hippo and TGF-beta signalling pathways. Nat Commun. 2016 Mar 31 [PMID:27030211] (WB, Human) WB Human
Moreno P, Lara-Chica M, Soler-Torronteras R et al. The Expression of the Ubiquitin Ligase SIAH2 (Seven In Absentia Homolog 2) Is Increased in Human Lung Cancer. PLoS ONE. 2015 Nov 20 [PMID:26580787] (IHC-P, Human) IHC-P Human
Wong CS, Chen A, Liu MC et al. Loss of Siah2 does not impact angiogenic potential of murine endothelial cells. Microvasc. Res. 2015 Aug 11 [PMID:26275748] (WB, Mouse) WB Mouse
Qin R, Smyrk TC, Reed NR et al. Combining clinicopathological predictors and molecular biomarkers in the oncogenic K-RAS/Ki67/HIF-1alpha pathway to predict survival in resectable pancreatic cancer Br. J. Cancer. 2015 Jan 13 [PMID:25584484] (IHC-P, Human) IHC-P Human
Tanaka T, Li T, Urata Y et al. Increased expression of PHD3 represses the HIF-1 signaling pathway and contributes to poor neovascularization in pancreatic ductal adenocarcinoma. J. Gastroenterol. 2014 Dec 27 [PMID:25542265] (IHC-P, Human) IHC-P Human
Ma B, Chen Y, Chen L et al. Hypoxia regulates Hippo signalling through the SIAH2 ubiquitin E3 ligase. Nat. Cell Biol. 2015 Jan 01 [PMID:25438054] (IHC-P, Human) IHC-P Human
Rizzardi AE, Rosener NK, Koopmeiners JS et al. Evaluation of protein biomarkers of prostate cancer aggressiveness. BMC Cancer 2014 Apr 09 [PMID:24708576] (IHC-P, Human) IHC-P Human
Qi J, Tripathi M, Mishra R et al. The e3 ubiquitin ligase siah2 contributes to castration-resistant prostate cancer by regulation of androgen receptor transcriptional activity Cancer Cell 2013 Mar 18 [PMID:23518348] (IHC, Human) IHC Human
Chan P, Moller A, Liu MC et al. The expression of the ubiquitin ligase SIAH2 (seven in absentia homolog 2) is mediated through gene copy number in breast cancer and is associated with a basal-like phenotype and p53 expression Breast Cancer Res 2011 Feb [PMID:21306611] (IHC, Human) IHC Human
Show All 12 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SIAH2 Antibody (NB110-88113). (Showing 1 - 1 of 1 FAQs).

  1. Hi, we are trying to find out if the antibody NB110-88113 (SIAH2 Antibody (24E6H3) 0.5ml Cancer Marker, Stem Cell Marker) will be useful for our application. We want to try to look at Siah (sina in Drosophila) in drosophila larval brains, more specifically in the Drosophila neural stem cells. As in the webpage it says that this antibody recognizes Drosophila Sina we would like to ask if this antibody was tested in Drosophila tissues or Drosophila Cells.
    • Our SIAH2 antibody with catalogue number NB110-88113 is covered by our 100% guarantee for the detection of SIAH2 in samples from Drosophila by Western blotting, Flow Cytometry, Immunofluorescence, and Immunohistochemistry with both frozen and paraffin-embedded samples. For further information on our guarantee please see the following link: Quality Assurance. The immunogen used to generate this antibody was a synthetic peptide made to an N-terminal portion (within residues 1-100) of the drosophila SINA protein (Swiss-Prot# P21461). Although we show data from human samples on our webpage for this antibody, we unfortunately do not have any images from Drosophila samples that I can share with you at the present time.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol SIAH2

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