SIAH2 Antibody (24E6H3) - BSA Free

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Staining of SIAH in human colorectal adenocarcinoma using NB110-88113. Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.

Immunocytochemistry/Immunofluorescence: SIAH2 Antibody (24E6H3) [NB110-88113] - Staining of SIAH in ovarian carcinoma (OVCR3) cells using NB110-88113.

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Strong SIAH2 staining in all nuclei in this basal-like breast carcinoma. Image collected and cropped by CiteAb from the following publication (breast-cancer-research.biomedcentral.com/articles/10.1186/bcr2828), licensed under a CC-BY license.

Flow (Intracellular): SIAH2 Antibody (24E6H3) [NB110-88113] - Human HepG2 cell line was stained with Mouse Anti- SIAH2 Monoclonal Antibody (Catalog# NB110-88113, orange), or Mouse IgG1 isotype control (Catalog# MAB002, blue) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog# FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog# FC005).

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Moderate to strong staining of SIAH2 in the nucleus of a small proportion of the cell in a high nuclear grade ductal carcinoma in situ with comedo necrosis. Image collected and cropped by CiteAb from the following publication (breast-cancer-research.biomedcentral.com/articles/10.1186/bcr2828), licensed under a CC-BY license.

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Occasional weak to moderate SIAH2 (arrows) staining in a luminal type ductal carcinoma. Image collected and cropped by CiteAb from the following publication (breast-cancer-research.biomedcentral.com/articles/10.1186/bcr2828), licensed under a CC-BY license.

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Immunohistochemistry of candidate biomarkers in prostate cancer. Representative immunohistochemical staining of ACPP, ADAM9, ALDH1A2, CASR, CCND1, CCPG1, CD34, CD44, CD44v6, CHGA, CHMP1A, EI24, ENO2, GADD45B, HA, HAS2, HES6, HMMR, HOXC6, HYAL1, IGF1, IQCK, MAP4K4, MKI67, PAGE4, PLIN2, PTEN, SIAH2, SMAD4, SOX9, SPP1, SYP, & TP53 from prostate cancer tissue microarrays. Scale bar represents 50 μm. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-244), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Po, Dr, Mu(-)
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 24E6H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

SIAH2 Antibody (24E6H3) - BSA Free Summary

• Description: Novus Biologicals Mouse SIAH2 Antibody (24E6H3) - BSA Free (NB110-88113) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-SIAH2 Antibody: Cited in 19 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide made to an N-terminal portion of the drosophila SINA protein (within residues 1-100). [Swiss-Prot# P21461]
• Localization: Nuclear, Cytoplasmic
• Specificity: This is specific for human SIAH2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIAH2
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry 1:10
  • Immunocytochemistry/ Immunofluorescence 1:10
  • Immunohistochemistry 1:40-1:50
  • Immunohistochemistry-Frozen 1:40-1:50
  • Immunohistochemistry-Paraffin 1:40-1:50
  • Western Blot
• Control:

  SIAH2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: 0.1% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for SIAH2 Antibody (24E6H3) - BSA Free

• E3 ubiquitin-protein ligase SIAH2
• EC 6.3.2
• EC 6.3.2.-
• hSiah2
• seven in absentia (Drosophila) homolog 2
• seven in absentia homolog 2 (Drosophila)
• Seven in absentia homolog 2
• siah-2

Background

The Drosophila SINA (seven in absentia) protein is an E3 ubiquitin ligase component of the RAS signal transduction pathway. The RAS signal pathway controls cell proliferation, differentiation, and survival, and regulation of this pathway is critical for normal development. In Drosophila SINA serves as a downstream gatekeeper required for proper RAS signal transduction. Similarly to SINA in Drosophila, the human protein SIAH has also been shown to be required for oncogenic RAS signaling in cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIAH2 Antibody (NB110-88113)(20)

Publications using NB110-88113

• Zhu Y, Liu Q, Luo Q et al. Hypoxia Dynamically Regulates DBC1 Ubiquitination and Stability by SIAH2 and OTUD5 in Breast Cancer Progression bioRxiv 2022-01-01 (IF/IHC, Human)
• Liu Q, Luo Q, Feng J et al. Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression eLife 2022-08-01 [PMID: 35913115] (Flow Cytometry, Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Human)
• Yan T, Zhou D, Shi Y et al. Targeting ADT-Induced Activation of the E3 Ubiquitin Ligase Siah2 to Delay the Occurrence of Castration-Resistant Prostate Cancer Frontiers in Oncology 2021-04-16 [PMID: 33937036] (Flow Cytometry, Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Human)
• Hughes SE, Hemenway E, Guo F The E3 ubiquitin ligase Sina regulates the assembly and disassembly of the synaptonemal complex in Drosophila females PLoS Genet. 2019-05-01 [PMID: 31107865] (ICC/IF, Drosophila)
• Ma B, Cheng H, Mu C et al. The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression Nat Commun 2019-03-04 [PMID: 30833558] (IF/IHC, Human)
• Jing Y, Nguyen MM, Wang D et al. DHX15 promotes prostate cancer progression by stimulating Siah2-mediated ubiquitination of androgen receptor. Oncogene 2018-02-01 [PMID: 28991234] (IF/IHC, Human)
• van Reesema LL, Zheleva V, Winston JS et al. SIAH and EGFR, Two RAS Pathway Biomarkers, are Highly Prognostic in Locally Advanced and Metastatic Breast Cancer. EBioMedicine. 2016-08-14 [PMID: 27569656] (IF/IHC, Human)
• van der Willik KD, Timmermans MM. SIAH2 protein expression in breast cancer is inversely related with ER status and outcome to tamoxifen therapy. Am J Cancer Res. 2016-02-01 [PMID: 27186402] (IHC-P, Human)
• Ma B, Cheng H, Gao R et al. Zyxin-Siah2-Lats2 axis mediates cooperation between Hippo and TGF-beta signalling pathways. Nat Commun. 2016-03-31 [PMID: 27030211] (WB, Human)
• Moreno P, Lara-Chica M, Soler-Torronteras R et al. The Expression of the Ubiquitin Ligase SIAH2 (Seven In Absentia Homolog 2) Is Increased in Human Lung Cancer. PLoS ONE. 2015-11-20 [PMID: 26580787] (IHC-P, Human)
• Wong CS, Chen A, Liu MC et al. Loss of Siah2 does not impact angiogenic potential of murine endothelial cells. Microvasc. Res. 2015-08-11 [PMID: 26275748] (WB, Mouse)
  
  Details:
  SIAH2 antibody (clone 24E6H3, NB110-88113) was used for WB on lysates of endothelial cells isolated from lungs of wildtype (WT) and Siah2?/? (KO) mice.
• Qin R, Smyrk TC, Reed NR et al. Combining clinicopathological predictors and molecular biomarkers in the oncogenic K-RAS/Ki67/HIF-1alpha pathway to predict survival in resectable pancreatic cancer Br. J. Cancer. 2015-01-13 [PMID: 25584484] (IHC-P, Human)
• Tanaka T, Li T, Urata Y et al. Increased expression of PHD3 represses the HIF-1 signaling pathway and contributes to poor neovascularization in pancreatic ductal adenocarcinoma. J. Gastroenterol. 2014-12-27 [PMID: 25542265] (IHC-P, Human)
• Chen Anna, Wong Christina S F, Liu Mira C P et al. The ubiquitin ligase Siah is a novel regulator of Zeb1 in breast cancer. Oncotarget 2015-01-01 [PMID: 25528765] (WB, Human)
• Ma B, Chen Y, Chen L et al. Hypoxia regulates Hippo signalling through the SIAH2 ubiquitin E3 ligase. Nat. Cell Biol. 2015-01-01 [PMID: 25438054] (IHC-P, Human)
  
  Details:
  SIAH2 antibody (clone 24E6H3) used for IHC-P on TMAs of invasive breast carcinoma and analysable cases of normal breast tissue - heat induced antigen retrieval with 10 mM sodium citrate buffer pH 6.0 for 20 min at 95C, blocking with 5% goat serum for 1 hour, endogenous peroxidase activity blocked with30 min incubation in 0.3% H2O2, primary used at 1:40 dilution, detection via HRP-conjugated secondary and DAB Substrate Kit from MaiXin Bio (Figure 4i).
• Rizzardi AE, Rosener NK, Koopmeiners JS et al. Evaluation of protein biomarkers of prostate cancer aggressiveness. BMC Cancer 2014-04-05 [PMID: 24708576] (IHC-P, Human)
• Qi J, Tripathi M, Mishra R et al. The e3 ubiquitin ligase siah2 contributes to castration-resistant prostate cancer by regulation of androgen receptor transcriptional activity Cancer Cell 2013-03-18 [PMID: 23518348] (IF/IHC, Human)
• Chan P, Moller A, Liu MC et al. The expression of the ubiquitin ligase SIAH2 (seven in absentia homolog 2) is mediated through gene copy number in breast cancer and is associated with a basal-like phenotype and p53 expression Breast Cancer Res 2011-02-01 [PMID: 21306611] (IF/IHC, Human)
• Ahmed, AU et al. Effect of disrupting seven-in-absentia homolog 2 function on lung cancer cell growth. J Natl Cancer Inst;100(22):1606-29. 2008-11-19 [PMID: 19001609] (IF/IHC, WB, Human)
• Schmidt, RL et al. Inhibition of RAS-mediated transformation and tumorigenesis by targeting the downstream E3 ubiquitin ligase seven in absentia homologue. Cancer Res;67(24):11798-810. 2007-12-15 [PMID: 18089810] (WB, FLOW, IF/IHC, ICC/IF, Human)

FAQs for SIAH2 Antibody (NB110-88113). (Showing 1 - 1 of 1 FAQs).

1. Hi, we are trying to find out if the antibody NB110-88113 (SIAH2 Antibody (24E6H3) 0.5ml Cancer Marker, Stem Cell Marker) will be useful for our application. We want to try to look at Siah (sina in Drosophila) in drosophila larval brains, more specifically in the Drosophila neural stem cells. As in the webpage it says that this antibody recognizes Drosophila Sina we would like to ask if this antibody was tested in Drosophila tissues or Drosophila Cells.
   • Our SIAH2 antibody with catalogue number NB110-88113 is covered by our 100% guarantee for the detection of SIAH2 in samples from Drosophila by Western blotting, Flow Cytometry, Immunofluorescence, and Immunohistochemistry with both frozen and paraffin-embedded samples. For further information on our guarantee please see the following link: Quality Assurance. The immunogen used to generate this antibody was a synthetic peptide made to an N-terminal portion (within residues 1-100) of the drosophila SINA protein (Swiss-Prot# P21461). Although we show data from human samples on our webpage for this antibody, we unfortunately do not have any images from Drosophila samples that I can share with you at the present time.

Bioinformatics

• Gene Symbol: SIAH2
• Entrez:
  • 6478(Human)
  • 20439(Mouse)