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![Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596] - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly speci](http://images.novusbio.com/fullsize/SIAH1-2-Antibody-8G7H12-Western-Blot-NB110-93596-img0002.jpg "Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596] - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly speci")
![Immunocytochemistry/Immunofluorescence: SIAH1 Antibody [NB300-974] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial/cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-974-img0001.jpg "Immunocytochemistry/Immunofluorescence: SIAH1 Antibody [NB300-974] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial/cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Western Blot: SIAH1 Antibody [NBP2-93864] - Analysis of extracts of mouse kidney, using SIAH1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Western-Blot-NBP2-93864-img0005.jpg "Western Blot: SIAH1 Antibody [NBP2-93864] - Analysis of extracts of mouse kidney, using SIAH1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: SIAH1 Antibody [NBP2-93864] - Immunofluorescence analysis of L929 cells using SIAH1 Rabbit pAb (NBP2-93864) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93864-img0006.jpg)
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-93864] - Rat brain using SIAH1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-93864-img0004.jpg)
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-93864] - Human colon carcinoma using SIAH1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-93864-img0002.jpg)
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-93864] - Mouse spleen using SIAH1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-93864-img0003.jpg)
![Western Blot: SIAH1 Antibody [NBP2-20356] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SIAH1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Western-Blot-NBP2-20356-img0006.jpg "Western Blot: SIAH1 Antibody [NBP2-20356] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SIAH1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356] - Human lung Papillary adenocarcinoma, using SIAH1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-20356-img0004.jpg)
![Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-28820241246162.jpg "Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Immunocytochemistry analysis of A549 cells labeling SIAH1 with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-29202410234222.jpg)
![Western Blot: SIAH1 Antibody (2C5) [H00006477-M02] - SIAH1 monoclonal antibody (M02), clone 2C5. Analysis of SIAH1 expression in Jurkat.](http://images.novusbio.com/fullsize/SIAH1-Antibody-2C5-Western-Blot-H00006477-M02-img0002.jpg "Western Blot: SIAH1 Antibody (2C5) [H00006477-M02] - SIAH1 monoclonal antibody (M02), clone 2C5. Analysis of SIAH1 expression in Jurkat.")
![Sandwich ELISA: SIAH1 Antibody (2C5) [H00006477-M02] - Detection limit for recombinant GST tagged SIAH1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SIAH1-Antibody-2C5-Sandwich-ELISA-H00006477-M02-img0001.jpg)
![Western Blot: SIAH1 Antibody [NBP3-30880] - Western blot using Novus Biologicals' NBP3-30880, rabbit polyclonal at 1 ug/ml on human brain lysate (20 ug/lane). Blots were developed with goat anti-MIg (1:30k) and Pierce’s Supersignal West Femto system.](http://images.novusbio.com/fullsize/nbp3-30880_rabbit-siah1-pab-81120242059429.jpg "Western Blot: SIAH1 Antibody [NBP3-30880] - Western blot using Novus Biologicals' NBP3-30880, rabbit polyclonal at 1 ug/ml on human brain lysate (20 ug/lane). Blots were developed with goat anti-MIg (1:30k) and Pierce’s Supersignal West Femto system.")
![Western Blot: SIAH1 Antibody [NBP3-30882] - Western blot using Novus Biologicals' NBP3-30882, affinity purified rabbit polyclonal at 1 ug/ml on human brain lysate (20 ug/lane). Blots were developed with goat anti-MIg (1:30k) and Pierce’s Supersignal West Femto system.](http://images.novusbio.com/fullsize/nbp3-30882_rabbit-siah1-pab-811202420592153.jpg "Western Blot: SIAH1 Antibody [NBP3-30882] - Western blot using Novus Biologicals' NBP3-30882, affinity purified rabbit polyclonal at 1 ug/ml on human brain lysate (20 ug/lane). Blots were developed with goat anti-MIg (1:30k) and Pierce’s Supersignal West Femto system.")
![Western Blot: SIAH1 Overexpression Lysate (Adult Normal) [NBP2-04239] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIAH1.](http://images.novusbio.com/fullsize/SIAH1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04239-img0001.jpg "Western Blot: SIAH1 Overexpression Lysate (Adult Normal) [NBP2-04239] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIAH1.")
![Western Blot: SIAH1 Overexpression Lysate (Adult Normal) [NBL1-15954] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIAH1.](http://images.novusbio.com/fullsize/SIAH1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15954-img0002.jpg "Western Blot: SIAH1 Overexpression Lysate (Adult Normal) [NBL1-15954] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIAH1.")
![SDS-Page: SIAH1 Protein [NBP2-23470]](http://images.novusbio.com/fullsize/SIAH1-Protein-SDS-Page-NBP2-23470-img0001.jpg "SDS-Page: SIAH1 Protein [NBP2-23470]")