Shugoshin Antibody

Western Blot: Shugoshin Antibody [NBP1-03361] - Detection of SGO1 in HeLa nuclear extract using NBP1-03361.

Immunocytochemistry/Immunofluorescence: Shugoshin Antibody [NBP1-03361] - Shugoshin antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: Shugoshin Antibody [NBP1-03361] - IHC analysis of formalin-fixed paraffin-embedded tissue section of infiltrating ductal carcinoma of human breast using rabbit polyclonal Shugoshin antibody (NBP1-03361) at 5 ug/ml concentration. The carcinoma cells depicted a moderate to strong nuclear with weak cytoplasmic immunopositivity of Shugoshin protein, whereas, the collagen fibers in the surrounding tumor stroma/connective tissue also developed a very weak/potentially nonspecific staining [Magnification 40X].

Immunohistochemistry-Paraffin: Shugoshin Antibody [NBP1-03361] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using rabbit polyclonal Shugoshin antibody (NBP1-03361) at 5 ug/ml concentration. The carcinoma cells depicted a distinct nuclear with weak cytoplasmic immuno-reactivity for Shugoshin protein [Magnification 40X]

Product Discontinued

Shugoshin Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of human Shugoshin (within residues 350-450). [Swiss-Prot# Q5FBB7]
• Localization: Nucleus. Centromere. Note= Localizes to the centromere. BUB1 is required for centromeric localization. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SGO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2 ug/ml
• Application Notes: This Shugoshin antibody is useful for Western blot, where a band is seen at ~64 kDa. In ICC/IF nuclear staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Shugoshin Antibody

• FLJ14230
• hSgo1
• NY-BR-85
• Serologically defined breast cancer antigen NY-BR-85
• SGO
• Sgo1
• shugoshin 1AB protein
• shugoshin 1CD protein
• shugoshin 1EF protein
• shugoshin 1GH protein
• shugoshin 1KL protein
• shugoshin-like 1 (S. pombe)
• shugoshin-like 1

Background

Shugoshin (SGOL) proteins provide essential protection to cohesin (a protein complex that hold sister chromatids together) from cleavage by separase enzyme during meiosis-I as well as from phosphorylation-mediated removal during mitotic cell division. SGOL forms a complex with phosphatase PP2A that dephosphorylates cohesion resulting in its retention at centromeres during the onset of meiotic anaphase and vertebrate mitotic prophase I. Shugoshin (Japanese for guardian spirit), was originally identified in budding/fission yeasts as protector of centromeric cohesion in meiosis and later on it was found to be highly conserved among eukaryotic organisms with mammalian SGOL family consisting of two members: SGOL1 and SGOL2. SGOL1 regulates accurate chromosome segregation during mitosis, whereas, SGOL2 plays a dual role in chromosome congression as well as centromeric protection of cohesion. Defects in chromosome cohesion system and mis-regulation of SGOL functions may lead to pathologies including cancer and infertility. SGO1 has been shown to implicate in human colon cancers and lentivirus-mediated siRNA interference targeting SGO-1 inhibits human NSCLC cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SGO1
• Entrez:
  • 151648(Human)
• Uniprot:
  • Q5FBB7(Human)