Shugoshin Antibody


Western Blot: Shugoshin Antibody [NBP1-03361] - Detection of SGO1 in HeLa nuclear extract using NBP1-03361.
Immunocytochemistry/ Immunofluorescence: Shugoshin Antibody [NBP1-03361] - Shugoshin antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight more
Immunohistochemistry-Paraffin: Shugoshin Antibody [NBP1-03361] - IHC analysis of formalin-fixed paraffin-embedded tissue section of infiltrating ductal carcinoma of human breast using rabbit polyclonal Shugoshin more
Immunohistochemistry-Paraffin: Shugoshin Antibody [NBP1-03361] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using rabbit polyclonal Shugoshin antibody more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
1 mg/ml

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

Shugoshin Antibody Summary

Synthetic peptide made to an internal portion of human Shugoshin (within residues 350-450). [Swiss-Prot# Q5FBB7]
Nucleus. Centromere. Note= Localizes to the centromere. BUB1 is required for centromeric localization. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate, pH 7-8
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
This Shugoshin antibody is useful for Western blot, where a band is seen at ~64 kDa. In ICC/IF nuclear staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
64 kDa

Reactivity Notes

Immunogen sequence has 53% homology to Mouse's Shugoshin/SGOL1 protein.

Alternate Names for Shugoshin Antibody

  • FLJ14230
  • hSgo1
  • NY-BR-85
  • Serologically defined breast cancer antigen NY-BR-85
  • SGO
  • Sgo1
  • shugoshin 1AB protein
  • shugoshin 1CD protein
  • shugoshin 1EF protein
  • shugoshin 1GH protein
  • shugoshin 1KL protein
  • shugoshin-like 1 (S. pombe)
  • shugoshin-like 1


Shugoshin (SGOL) proteins provide essential protection to cohesin (a protein complex that hold sister chromatids together) from cleavage by separase enzyme during meiosis-I as well as from phosphorylation-mediated removal during mitotic cell division. SGOL forms a complex with phosphatase PP2A that dephosphorylates cohesion resulting in its retention at centromeres during the onset of meiotic anaphase and vertebrate mitotic prophase I. Shugoshin (Japanese for guardian spirit), was originally identified in budding/fission yeasts as protector of centromeric cohesion in meiosis and later on it was found to be highly conserved among eukaryotic organisms with mammalian SGOL family consisting of two members: SGOL1 and SGOL2. SGOL1 regulates accurate chromosome segregation during mitosis, whereas, SGOL2 plays a dual role in chromosome congression as well as centromeric protection of cohesion. Defects in chromosome cohesion system and mis-regulation of SGOL functions may lead to pathologies including cancer and infertility. SGO1 has been shown to implicate in human colon cancers and lentivirus-mediated siRNA interference targeting SGO-1 inhibits human NSCLC cell growth.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for Shugoshin Antibody (NBP1-03361): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

DyLight 405 Labeled NBP1-03361V
FITC Labeled NBP1-03361F

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Contact Information

Product PDFs


Gene Symbol SGOL1

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