SGOL2 Antibody - BSA Free

Staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.

Analysis in human testis and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SGOL2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ESHSHSDQSSKTSLMSEMRNAQSIGRRWEKPSPSNVTERKKRGSSWESNNLSADTPCATVLDKQHISSPELNCNNEINGHTNETNTEMQRN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SGOL2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SGOL2 Protein (NBP1-83567PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SGOL2 Antibody - BSA Free

• FLJ25211
• MGC129665
• Sgo2
• shugoshin-2
• shugoshin-like 2 (S. pombe)
• Tripin
• TRIPINshugoshin-like 2

Background

Shugoshin-like 2 (SGO2) is a member of the shugoshin (SGO) family of proteins which plays a role in preventing premature release of the cohesin complex from centromeres during prophase and mitosis. The SGO family of proteins is evolutionarily conserved across eukaryotes and is central to the maintenance of genomic stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SGOL2 Antibody (NBP1-83567)(5)

Publications using NBP1-83567

• Mihalas B, Pieper G, Currie C et al. Age-dependent loss of cohesion protection in human oocytes bioRxiv 2023-01-14 [PMID: 38134935] (ICC/IF, Human)
  
  Details:
  Dilution 1:50
• Wu Z, Zhuo T, Li Z et al. High SGO2 predicted poor prognosis and high therapeutic value of lung adenocarcinoma and promoted cell proliferation, migration, invasion, and epithelial-to-mesenchymal transformation Journal of Cancer 2023-07-24 [PMID: 37576392] (Western Blot)
• Gantchev Jennifer, Martinez Villarreal Amelia, Xie Pingxing et al. The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL). Frontiers in Oncology 2019-05-31 [PMID: 31214493] (IHC-P, Human)
• Lindsey S, Byrnes D, Eller M et al. Potential Role of Meiosis Proteins in Melanoma Chromosomal Instability. J Skin Cancer 2013 [PMID: 23840955] (WB)
• Lindsey S, Byrnes D, Eller M et al. Potential role of meiosis proteins in melanoma chromosomal instability. J Skin Cancer. 2013 [PMID: 23840955] (WB)

Bioinformatics

• Gene Symbol: SGOL2