GIRK2 Antibody

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Western Blot: GIRK2 Antibody [NB100-74575] - Staining of Human Brain (Substantia Nigra) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GIRK2 Antibody Summary

Immunogen
Peptide with sequence C-SSKLNQHAELET corresponding to C-Terminus according to NP_002231.1.
Localization
Plasma membrane
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KCNJ6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 48 kDa band observed in human brain (hippocampus and substantia nigra) lysates (calculated MW of 48.5 kDa band according to NP_002231.1). Use in IHC, ICC/IF reported in scientific literature (PMID: 28398344).
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-74575 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GIRK2 Antibody

  • BIR1Kir3.2
  • G protein-activated inward rectifier potassium channel 2
  • GIRK-2
  • GIRK2KATP-2
  • hiGIRK2
  • Inward rectifier K(+) channel Kir3.2
  • inward rectifier potassium channel KIR3.2
  • KATP2human G protein coupled inward rectifier potassium channel 2 (hiGIRK2)10KCNJ7KIR3.2
  • MGC126596
  • Potassium channel, inwardly rectifying subfamily J member 6
  • potassium inwardly-rectifying channel, subfamily J, member 6

Background

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and may be involved in the regulation of insulin secretion by glucose. It associates with two other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GIRK2 Antibody (NB100-74575)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol KCNJ6
Entrez
Uniprot