Serum Response Factor SRF Antibody - BSA Free

Western Blot: Serum Response Factor SRF Antibody [NBP1-71927] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-SRF antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SRF was also immunoprecipitated by rabbit anti-SRF antibody NBP1-71928, which recognizes a downstream epitope.

Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody [NBP1-71927] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SRF used at a dilution of 1:1,000 (1ug/ml) (left) and used at a dilution of 1:1,000 (1ug/ml) (right) . Detection: DAB

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Serum Response Factor SRF Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Serum Response Factor SRF Antibody - BSA Free (NBP1-71927) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-Serum Response Factor SRF Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 25 and 75 of human Serum Response Factor using the numbering given in entry NP_003122.1 (GeneID 6722).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID:33260953)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500- 1:2000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Serum Response Factor SRF Antibody - BSA Free

• MCM1
• serum response factor (c-fos serum response element-binding transcriptionfactor)
• Serum Response Factor SRF
• serum response factor
• SRF

Background

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serum Response Factor SRF Antibody (NBP1-71927)(1)

Publication using NBP1-71927

• Watson RW, Azam H, Aura C et al. Inhibition of Serum Response Factor Improves Response to Enzalutamide in Prostate Cancer Cancers 2020-11-27 [PMID: 33260953] (ICC/IF, IF/IHC)

Bioinformatics

• Gene Symbol: SRF
• Uniprot:
  • NP_003122.1(Human)