Elk-1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Elk-1 Antibody [NBP2-38278] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278] - Staining of human pancreas shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278] - Analysis in human ovary and pancreas tissues using Anti-ELK1 antibody. Corresponding ELK1 RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278] - Staining of human ovary shows high expression.
Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278] - Staining in human ovary and pancreas tissues using anti-ELK1 antibody. Corresponding ELK1 RNA-seq data are presented for the same tissues.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Elk-1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: HPRPAVVLPSAAPAGAAAPPSGSRSTSPSPLEACLEAEEAGLPLQVILTPPEAPNLKSEELNVEPGLGRALPPEVKVEGPKEE
Specificity
Specificity of human Elk-1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ELK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Elk-1 Protein (NBP2-38278PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Rat (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Elk-1 Antibody

  • Elk1
  • Elk-1
  • ELK1, member of ETS oncogene family
  • ETS domain-containing protein Elk-1
  • ETS-like gene 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ELK1
Entrez
Uniprot