Serpin A8/Angiotensinogen Antibody

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Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6966) followed by ...read more
Serpin A8/Angiotensinogen was detected in perfusion fixed frozen sections of mouse intestine using Sheep Anti-Mouse Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6966) at 1.7 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Serpin A8/Angiotensinogen Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Serpin A8/Angiotensinogen
Asp25-Val477
Accession # AAH19496
Specificity
Detects mouse Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In direct ELISAs, approximately 24% cross-reactivity with recombinant human Angiotensinogen is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Serpin A1, rmSerpin C1, and rmSerpin F2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
AGT
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF6966 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A8/Angiotensinogen Antibody

  • AGT
  • alpha-1 antiproteinase, antitrypsin
  • Angiotensin II
  • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • Angiotensinogen
  • ANHU
  • Aogen
  • FLJ92595
  • FLJ97926
  • PAT
  • pre-angiotensinogen
  • serine (or cysteine) proteinase inhibitor
  • Serpin A8
  • SERPINA8angiotensin I

Background

Serpin A8 (serine proteinase inhibitor-clade A8; also angiotensinogen) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II and III. Ang II induces vascoconstriction and aldosterone release by acting on AT1 receptors, while Ang III drives aldosterone release. Ang I can be further processed by MME to generate Ang, a peptide that binds MAS1 on platelets, and promotes the release of NO, an antithrombotic agent. Mature mouse angiotensinogen is 453 aa in length (aa 25-477). It contains Ang I (aa 25-34) that is cleaved to create Ang II (aa 25-32), Ang III (aa 26-32) and Ang I (aa 25-31). Serpin A8/Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg. There is an alternative start site five aa upstream of the standard site. Over aa 25-477, mouse serpin A8 shares 86% and 61% aa identity with rat and human serpin A8, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin A8/Angiotensinogen Antibody (AF6966)(2)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol AGT
Uniprot