Serpin A8/Angiotensinogen Antibody

Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6966) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Serpin A8/Angiotensinogen at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Serpin A8/Angiotensinogen was detected in perfusion fixed frozen sections of mouse intestine using Sheep Anti-Mouse Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6966) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the brush border in intestinal epithelium. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin A8/Angiotensinogen Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Serpin A8/Angiotensinogen
  Asp25-Val477
  Accession # AAH19496
• Specificity: Detects mouse Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In direct ELISAs, approximately 24% cross-reactivity with recombinant human Angiotensinogen is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Serpin A1, rmSerpin C1, and rmSerpin F2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AGT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A8/Angiotensinogen Antibody

• AGT
• alpha-1 antiproteinase, antitrypsin
• Angiotensin II
• angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Angiotensinogen
• ANHU
• Aogen
• FLJ92595
• FLJ97926
• PAT
• pre-angiotensinogen
• serine (or cysteine) proteinase inhibitor
• Serpin A8
• SERPINA8angiotensin I

Background

Serpin A8 (serine proteinase inhibitor-clade A8; also angiotensinogen) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II and III. Ang II induces vascoconstriction and aldosterone release by acting on AT1 receptors, while Ang III drives aldosterone release. Ang I can be further processed by MME to generate Ang, a peptide that binds MAS1 on platelets, and promotes the release of NO, an antithrombotic agent. Mature mouse angiotensinogen is 453 aa in length (aa 25-477). It contains Ang I (aa 25-34) that is cleaved to create Ang II (aa 25-32), Ang III (aa 26-32) and Ang I (aa 25-31). Serpin A8/Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg. There is an alternative start site five aa upstream of the standard site. Over aa 25-477, mouse serpin A8 shares 86% and 61% aa identity with rat and human serpin A8, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin A8/Angiotensinogen Antibody (AF6966)(2)

Publications using AF6966

• Juretzko A, Steinbach A, Witte J et al. Renal angiotensin I-converting enzyme-deficient mice are protected against aristolochic acid nephropathy Pflugers Archiv : European journal of physiology 12/15/2022 [PMID: 36520238]
• BL Phipps, U Suwannasua, J Lucero, NA Mitchell, AK Lund Vehicle emissions-exposure alters expression of systemic and tissue-specific components of the renin-angiotensin system and promotes outcomes associated with cardiovascular disease and obesity in wild-type C57BL/6 male mice Toxicology reports, 2021-04-15;8(0):846-862. 2021-04-15 [PMID: 33948438] (IHC, Mouse)

Bioinformatics

• Gene Symbol: AGT
• Uniprot:
  • AAH19496()