Serpin A8/Angiotensinogen Antibody [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

Serpin A8/Angiotensinogen Antibody [Alexa Fluor® 532] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Serpin A8/Angiotensinogen
  Asp25-Val477
  Accession # AAH19496
• Specificity: Detects mouse Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In direct ELISAs, approximately 24% cross-reactivity with recombinant human Angiotensinogen is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Serpin A1, r
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A8/Angiotensinogen Antibody [Alexa Fluor® 532]

• AGT
• alpha-1 antiproteinase, antitrypsin
• Angiotensin II
• angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Angiotensinogen
• ANHU
• Aogen
• FLJ92595
• FLJ97926
• PAT
• pre-angiotensinogen
• serine (or cysteine) proteinase inhibitor
• Serpin A8
• SERPINA8angiotensin I

Background

Serpin A8 (serine proteinase inhibitor-clade A8; also angiotensinogen) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II and III. Ang II induces vascoconstriction and aldosterone release by acting on AT1 receptors, while Ang III drives aldosterone release. Ang I can be further processed by MME to generate Ang, a peptide that binds MAS1 on platelets, and promotes the release of NO, an antithrombotic agent. Mature mouse angiotensinogen is 453 aa in length (aa 25-477). It contains Ang I (aa 25-34) that is cleaved to create Ang II (aa 25-32), Ang III (aa 26-32) and Ang I (aa 25-31). Serpin A8/Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg. There is an alternative start site five aa upstream of the standard site. Over aa 25-477, mouse serpin A8 shares 86% and 61% aa identity with rat and human serpin A8, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.