Serpin A8/Angiotensinogen Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin A8/Angiotensinogen at approximately 64 kDa (as indicated) ...read more
Serpin A8/Angiotensinogen was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 1.7 µg/mL Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Serpin A8/Angiotensinogen Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A8/Angiotensinogen
Asp34-Ala485
Accession # ABM85122
Specificity
Detects human Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Serpin A8/Angiotensinogen is observed, and less than 5% cross-reactivity with recombinant human Serpin A1, A4, A5, F2, G1, I1, and I2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AGT
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 2-15 ug/mL
Publications
Read Publications using
AF3156 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A8/Angiotensinogen Antibody [Unconjugated]

  • AGT
  • alpha-1 antiproteinase, antitrypsin
  • Angiotensin II
  • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • Angiotensinogen
  • ANHU
  • Aogen
  • FLJ92595
  • FLJ97926
  • PAT
  • pre-angiotensinogen
  • serine (or cysteine) proteinase inhibitor
  • Serpin A8
  • SERPINA8angiotensin I

Background

Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors.  It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I.  This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II.  Ang II induces vascoconstriction and aldosterone release.  Mature human angiotensinogen is 452 aa in length (aa 34-485).  It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41).  Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg.  Over aa 34-485, human serpin A8 shares 61% aa identity with mouse serpin A8.        

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serpin A8/Angiotensinogen Antibody (AF3156)(3)

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Biotin BAF3156

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Bioinformatics

Gene Symbol AGT
Uniprot