Serpin A8/Angiotensinogen Antibody (9Q5D4)

Western Blot: Serpin A8/Angiotensinogen Antibody (9Q5D4) [NBP3-33190] - Western blot analysis of various lysates using Serpin A8/Angiotensinogen Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.

Western Blot: Serpin A8/Angiotensinogen Antibody (9Q5D4) [NBP3-33190] - Western blot analysis of lysates from Mouse liver, using Serpin A8/Angiotensinogen Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clone: 9Q5D4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Serpin A8/Angiotensinogen Antibody (9Q5D4) Summary

• Description: Novus Biologicals Rabbit Serpin A8/Angiotensinogen Antibody (9Q5D4) (NBP3-33190) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Serpin A8/Angiotensinogen (P01019).
  
  Sequence:
  MAPAGVSLRATILCLLAWAGLAAGDRVYIHPFHLVIHNESTCEQLAKANAGKPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRAAMVGML
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AGT
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Serpin A8/Angiotensinogen Antibody (9Q5D4)

• AGT
• alpha-1 antiproteinase, antitrypsin
• Angiotensin II
• angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Angiotensinogen
• ANHU
• Aogen
• FLJ92595
• FLJ97926
• PAT
• pre-angiotensinogen
• serine (or cysteine) proteinase inhibitor
• Serpin A8
• SERPINA8angiotensin I

Background

Angiotensin II is encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGT