Serpin A1c/alpha 1-Antitrypsin Antibody [Alexa Fluor® 488]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Serpin A1c/alpha 1-Antitrypsin Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Serpin A1c/ alpha 1-Antitrypsin
  Glu25-Lys413
  Accession # NP_033271
• Specificity: Detects mouse Serpin A1c/ alpha 1-Antitrypsin in direct ELISAs and Western blots. In direct ELISAs, approximately 70% cross-reactivity with recombinant mouse Serpin A1a is observed, and less than 3% cross-reactivity with recombinant human Serpin A1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

SerpinA1c (serine proteinase inhibitor-clade A 1c; also alpha-1 protease inhibitor 6 and alpha1 antitrypsin 1-3) is a secreted, 50-55 kDa glycoprotein member of the clade A-subfamily, serpin superfamily of protease inhibitors. There are multiple sources for serpinA1. The circulating form of serpinA1 is expressed by hepatocytes, while local production occurs in the bone marrow by osteoblasts, PMNs, T cells and B cells. SerpinA1 is a naturally occurring serine protease inhibitor. Its principal activity seems to be that of neutralizing neutrophil elastase, an activity that protects the elasticity of the lung during inflammation. It is also posited to play a role in bone marrow progenitor mobilization. Here, it promotes HPC proliferation by blocking cytokine degradation, and interferes with HPC migration by blocking protease cleavage of engaged cell adhesion molecules. Mature mouse serpinA1c is 389 amino acids (aa) in length (aa 25-413) (GenBank#:NP_033271) and contains one active enzymatic site (aa 67-410). Unlike human and rat, the mouse genus contains anywhere from one to seven serpinA1genes. Mus caroli (an Asian species) possesses one gene, Mus saxicola (a south asia species) possesses four genes, and Mus musculus possesses six or more distinct genes. Within Mus musculus, various strains have variable numbers of active genes. While highly homologous, the protein sequences are not identical, and differ most importantly in the reactive center loop region (aa 374-386). The protein referenced here is equivalent to alpha 1-PI-3 (Borriello, F & K.S. Krauter [1991] Proc. Natl. Acad. Sci. USA 88:9417). This gene has one isoform with minimal scattered substitutions and shows more that 98% aa sequence identity (GenBank #:Q00896). Over aa 25-413, serpinA1c/ alpha 1-IP-3 shares 97% and 95% aa sequence identity with serpinA1a/ alpha 1-PI-1 and serpinA1b/ alpha 1-IP-2, respectively. It also shares 77% and 64% aa sequence identity with rat and human serpinA1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.