Separase Antibody (XJ11-1B12)

Images

 
Western Blot: Separase Antibody (XJ11-1B12) [NB100-439] - Separase detected in 5C6 cell lysates using NB 100-439. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
XJ11-1B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.9 mg/ml

Separase Antibody (XJ11-1B12) Summary

Immunogen
Maltose-Binding Protein fusion of a C-terminal fragment of human Separase (residues 1866-1996). [UniProt# Q14674]
Localization
Nuclear and Centrosomal
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ESPL1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
Application Notes
This Separase (XJ11-1B12) antibody is useful for Western Blot, where a band is seen at ~220 kDa (full-length form) and ~65 kDa (auto-processed form).
Publications
Read Publication using NB100-439.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
0.9 mg/ml
Purity
Protein G purified

Alternate Names for Separase Antibody (XJ11-1B12)

  • Caspase-like protein ESPL1
  • EC 3.4.22.49
  • ESP1extra spindle poles like 1
  • extra spindle pole bodies homolog 1 (S. cerevisiae)
  • extra spindle poles like 1 (S. cerevisiae)
  • Extra spindle poles-like 1 protein
  • FLJ46492
  • KIAA0165separin
  • Separase

Background

In normal cellular proliferation, the maintenance of constant centriole numbers is strongly associated with cell cycle process and the disengagement of mother-daugther centriole (a requirement for duplication of centriole) is facilitated by separase (ESPL1, a cysteine endopeptidase) mediated proteolytic cleavage of cohesin, a "glue" protein complex which is also essential to sister-chromatide cohesion. Separase is a caspase-like protease which plays a key role in chromosome segregation by cleaving SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle phases, separase is kept inactivated, and its proteolytic activity is tightly regulated by multiple inhibitory mechanisms combining securin binding, CyclinB1/Cdk1 mediated serine residue phosphorylation (pSer1226), PP2A binding and autocatalytic cleavage. Ectopic activation of separase proteolytic activity results in premature sister-chromatide separation/centriole disengagement and separase overexpression has been shown to induce aneuploidy and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Separase Antibody (NB100-439)(1)

We have publications tested in 1 confirmed species: Human.


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-439AF405
Alexa Fluor 488 Labeled NB100-439AF488
Alexa Fluor 647 Labeled NB100-439AF647
Alexa Fluor 700 Labeled NB100-439AF700
Biotin Labeled NB100-439B
DyLight 405 Labeled NB100-439V
DyLight 488 Labeled NB100-439G
DyLight 550 Labeled NB100-439R
DyLight 650 Labeled NB100-439C
HRP Labeled NB100-439H

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Bioinformatics

Gene Symbol ESPL1