Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1% Sodium Azide
Protein G purified
Alternate Names for Separase Antibody (XJ11-1B12)
Caspase-like protein ESPL1
ESP1extra spindle poles like 1
extra spindle pole bodies homolog 1 (S. cerevisiae)
extra spindle poles like 1 (S. cerevisiae)
Extra spindle poles-like 1 protein
In normal cellular proliferation, the maintenance of constant centriole numbers is strongly associated with cell cycle process and the disengagement of mother-daugther centriole (a requirement for duplication of centriole) is facilitated by separase (ESPL1, a cysteine endopeptidase) mediated proteolytic cleavage of cohesin, a "glue" protein complex which is also essential to sister-chromatide cohesion. Separase is a caspase-like protease which plays a key role in chromosome segregation by cleaving SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle phases, separase is kept inactivated, and its proteolytic activity is tightly regulated by multiple inhibitory mechanisms combining securin binding, CyclinB1/Cdk1 mediated serine residue phosphorylation (pSer1226), PP2A binding and autocatalytic cleavage. Ectopic activation of separase proteolytic activity results in premature sister-chromatide separation/centriole disengagement and separase overexpression has been shown to induce aneuploidy and carcinogenesis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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