NET1 Antibody (CL3063) - Azide and BSA Free

Staining of human kidney shows no positivity in cells in tubules or glomeruli as expected.

Staining of human placenta shows weak membranous positivity in trophoblastic cells.

Staining of human prostate shows strong positivity in sympathetic peripheral nerves.

Staining of human locus coeruleus shows strong cytoplasmic positivity in noradrenaline neurons.

Staining of mouse brain shows strong positivity in noradrenergic fibers in the cerebral cortex.

Staining of mouse brain shows strong positivity in noradrenergic fibers in the hippocampus.

Staining of rat pons shows strong positivity in noradrenaline neurons in the locus coeruleus.

Staining of rat cerebellum shows strong positivity in noradrenergic fibers.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: CL3063
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NET1 Antibody (CL3063) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of P23975, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NET1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for NET1 Antibody (CL3063) - Azide and BSA Free

• ARHGEF8neuroepithelial cell-transforming gene 1 protein
• guanine nucleotide regulatory protein (oncogene)
• NET1A
• neuroepithelial cell transforming 1
• neuroepithelial cell transforming gene 1
• neuroepithelioma transforming gene 1
• p65 Net1 proto-oncogene protein
• Proto-oncogene p65 Net1
• Rho guanine nucleotide exchange factor (GEF) 8
• Rho guanine nucleotide exchange factor 8
• small GTP-binding protein regulator

Background

NET1 is a guanyl nucleotide exchange factor for RhoA GTPase. It may be involved in signal transduction, in the activation of the SAPK/JNK pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NET1