Separase Antibody (XJ11-1B12) [DyLight 550]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
XJ11-1B12
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

Separase Antibody (XJ11-1B12) [DyLight 550] Summary

Immunogen
Maltose-Binding Protein fusion of a C-terminal fragment of human Separase (residues 1866-1996). [UniProt# Q14674]
Localization
Nuclear and Centrosomal
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ESPL1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This Separase (XJ11-1B12) antibody is useful for Western Blot, where a band is seen at ~220 kDa (full-length form) and ~65 kDa (auto-processed form).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Separase Antibody (XJ11-1B12) [DyLight 550]

  • Caspase-like protein ESPL1
  • EC 3.4.22.49
  • ESP1extra spindle poles like 1
  • extra spindle pole bodies homolog 1 (S. cerevisiae)
  • extra spindle poles like 1 (S. cerevisiae)
  • Extra spindle poles-like 1 protein
  • FLJ46492
  • KIAA0165separin
  • Separase

Background

Separase is a cysteine protease that is essential for mitotic progression by separating sister chromatids. Each cell must receive one chromatid of every chromosome, during mitosis. Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys cohesin by means of cleavage, allowing the chromatids to separate and divide with the cell. Separase activity is highly regulated. It not only cleaves cohesin at the onset of anaphase but also cleaves itself, promoting down-regulation of separase after anaphase. Should a human cell become an aneuploid (one too many or too few chromatids), the embryo most likely will not survive. Should the embryo survive, it will most likely develop severe birth defects or later develop malignant cancers. Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Separase Antibody (NB100-439R) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-439B
DyLight 405 Labeled NB100-439V
DyLight 488 Labeled NB100-439G
DyLight 650 Labeled NB100-439C
HRP Labeled NB100-439H

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Bioinformatics Tool for Separase Antibody (NB100-439R)

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Bioinformatics

Gene Symbol ESPL1