SENP3 Antibody

Western Blot: SENP3 Antibody [NBP2-20294] - Sample (30 ug of whole cell lysate) A: Raji B: K562 C: THP-1 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: SENP3 Antibody [NBP2-20294] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: SENP3 Antibody [NBP2-20294] - Human breast cancer, using SENP3 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SENP3 Antibody Summary

• Description: Novus Biologicals Rabbit SENP3 Antibody (NBP2-20294) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human SENP3. The exact sequence is proprietary.
• Localization: Nucleus; nucleolus
• Predicted Species: Mouse (100%), Rat (99%), Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SENP3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SENP3 Antibody

• DKFZP586K0919
• DKFZp762A152
• EC 3.4.22
• EC 3.4.22.-
• sentrin/SUMO-specific protease 3
• Sentrin/SUMO-specific protease SENP3
• sentrin-specific protease 3
• SMT3IP1
• SSP3DKFZp586K0919
• SUMO1/sentrin/SMT3 specific peptidase 3
• SUMO1/sentrin/SMT3 specific protease 3
• SUMO-1-specific protease 3
• SUSP3

Background

The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP3, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates (Di Bacco et al., 2006 [PubMed 16738315]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SENP3
• Uniprot:
  • Q9H4L4(Human)