Semaphorin 4D/CD100 Antibody (A8) - Azide and BSA Free

Flow Cytometry: Semaphorin 4D/CD100 Antibody (A8) - Azide and BSA Free [NBP2-36736] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-25198. Surface Staining of CD100 in 10^6 human lymphocytes using 10 ul (0.25 ug) of CD100 antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents CD100 antibody. surface staining flow kit was used for this test (cells were not fixed for testing).

Flow (Cell Surface): Semaphorin 4D/CD100 Antibody (A8) - Azide and BSA Free [NBP2-36736] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-25198. Staining of CD100 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents antibody.

Flow Cytometry: Semaphorin 4D/CD100 Antibody (A8) - Azide and BSA Free [NBP2-36736] - Cell surface analysis of CD100 in 10^6 human lymphocytes using 0.25 ug of NBP2-25198. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD100 antibody. Goat anti-mouse IgG PE conjugated secondary was used in this test.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

Semaphorin 4D/CD100 Antibody (A8) - Azide and BSA Free Summary

• Additional Information: Clone A8 was used by HLDA to establish CD designation.
• Immunogen: PHA-activated human lymphocytes were used as the immunogen for the CD100 antibody.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SEMA4D
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Semaphorin 4D/CD100 Antibody (A8) - Azide and BSA Free

• A8
• BB18
• CD100 antigen
• CD100
• CD100M-sema-G
• COLL-4
• FLJ33485
• FLJ34282
• FLJ39737
• FLJ46484
• immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
• MGC169138
• MGC169141
• M-sema-G
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
• SEMA4D
• SEMAJ
• Semaphorin 4D
• semaphorin-4D

Background

CD100 commonly known as SEMA4D is a cell surface protein of resting T cells involved in lymphocyte activation. An 862 amino acid signaling growth factor CD100 belongs to neuro-semaphorin family of proteins with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. Activation of plexin-B1 by CD100 induces different biological responses through the differential association of its receptor with ErbB-2 and Met. CD100 regulates certain immune responses by mediating in vitro and in vivo allo-geneic responses by modulating T cell-APC interactions and induces B cells aggregation, differentiation and improves their viability in vitro. Expression of CD100 on the surface of activated NK cells increases their cytotoxicity and mediates the killing of target cells by interacting with CD72. It is an important functional protein and a novel leukocyte semaphorin, of both the immune system, and the nervous system and is widely expressed in most of the tissues both hematopoietic and nonhematopoietic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA4D