PTK9 Antibody

Images

 
Western Blot: PTK9 Antibody [NBP1-90297] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: PTK9 Antibody [NBP1-90297] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Western Blot: PTK9 Antibody [NBP1-90297] - Analysis in human cell line A-431.
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Simple Western: PTK9 Antibody [NBP1-90297] - Simple Western lane view shows a specific band for PTK9 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using ...read more
Simple Western: PTK9 Antibody [NBP1-90297] - Electropherogram image(s) of corresponding Simple Western lane view. PTK9 antibody was used at 1:40 dilution on RT-4 and U-251MG lysate(s).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PTK9 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AEEELRQIKINEVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TWF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:40
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
PTK9 Protein (NBP1-90297PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PTK9 Antibody

  • A6 protein tyrosine kinase
  • A6
  • Protein A6
  • Protein tyrosine kinase 9MGC23788
  • PTK9 protein tyrosine kinase 9
  • PTK9MGC41876
  • twinfilin, actin-binding protein, homolog 1 (Drosophila)
  • twinfilin-1

Background

PTK9 encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TWF1