Semaphorin 4D/CD100 Antibody (A8)

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Flow Cytometry: Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-25198. Surface Staining of CD100 in 10^6 human lymphocytes using 10 ul (0.25 ug) of CD100 ...read more
Flow Cytometry: Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198] - Cell surface analysis of CD100 in 10^6 human lymphocytes using 0.25 ug of NBP2-25198. Shaded histogram represents cells without antibody; green represents ...read more
Flow (Cell Surface): Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-25198. Staining of CD100 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, Flow-CS, IP
Clone
A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Semaphorin 4D/CD100 Antibody (A8) Summary

Immunogen
PHA-activated human lymphocytes were used as the immunogen for the CD100 antibody.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SEMA4D
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
  • Flow (Cell Surface)

Reactivity Notes

This product reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
CD100 antibody at 0.5 mg/ml in PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Semaphorin 4D/CD100 Antibody (A8)

  • A8
  • BB18
  • CD100 antigen
  • CD100
  • CD100M-sema-G
  • COLL-4
  • FLJ33485
  • FLJ34282
  • FLJ39737
  • FLJ46484
  • immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
  • MGC169138
  • MGC169141
  • M-sema-G
  • sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
  • SEMA4D
  • SEMAJ
  • Semaphorin 4D
  • semaphorin-4D

Background

CD100 commonly known as SEMA4D is a cell surface protein of resting T cells involved in lymphocyte activation. An 862 amino acid signaling growth factor CD100 belongs to neuro-semaphorin family of proteins with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. Activation of plexin-B1 by CD100 induces different biological responses through the differential association of its receptor with ErbB-2 and Met. CD100 regulates certain immune responses by mediating in vitro and in vivo allo-geneic responses by modulating T cell-APC interactions and induces B cells aggregation, differentiation and improves their viability in vitro. Expression of CD100 on the surface of activated NK cells increases their cytotoxicity and mediates the killing of target cells by interacting with CD72. It is an important functional protein and a novel leukocyte semaphorin, of both the immune system, and the nervous system and is widely expressed in most of the tissues both hematopoietic and nonhematopoietic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP2-25198AF488
Alexa Fluor 647 Labeled NBP2-25198AF647
Allophycocyanin Labeled NBP2-25198APC
DyLight 350 Labeled NBP2-25198UV
DyLight 405 Labeled NBP2-25198V
DyLight 488 Labeled NBP2-25198G
DyLight 550 Labeled NBP2-25198R
DyLight 650 Labeled NBP2-25198C
DyLight 680 Labeled NBP2-25198FR
DyLight 755 Labeled NBP2-25198IR
PE Labeled NBP2-25198PE
PerCP Labeled NBP2-25198PCP

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Bioinformatics

Gene Symbol SEMA4D
Entrez
Uniprot