Seladin 1 Antibody

Western Blot: Seladin 1 Antibody [H00001718-B01P] - Analysis of DHCR24 expression in transfected 293T cell line by DHCR24 polyclonal antibody. Lane 1: DHCR24 transfected lysate(56.76 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Seladin 1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: DHCR24 (NP_055577.1, 1 a.a. - 516 a.a.) full-length human protein. MEPAVSLAVCALLFLLWVRLKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKTHKNIMINLMDILEVDTKKQIVRVEPLVTMGQVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRCTPSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICAKFTHESQRQENHFVEGLLYSLDEAVIMTGVMTDEAEPSKLNSIGNYYKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFILPSQPGLVHPKGNEAELYIDIGAYGEPRVKHFEARSCMRQLEKFVRSVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAARH
• Specificity: DHCR24 - 24-dehydrocholesterol reductase,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: DHCR24
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Seladin 1 Antibody

• 24-dehydrocholesterol reductaseNbla03646
• DCE
• desmosterol-to-cholesterol enzyme
• Diminuto/dwarf1 homolog
• EC 1.3.1.72
• KIAA0018SELADIN1
• seladin 1,3-beta-hydroxysterol delta-24-reductase
• seladin-1
• selective AD indicator 1,3 beta-hydroxysterol delta 24-reductase

Background

This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Seladin 1 Antibody (H00001718-B01P)(1)

Publication using H00001718-B01P

• Gilk SD, Cockrell DC, Luterbach C et al. Bacterial colonization of host cells in the absence of cholesterol. PLoS Pathog. 2013-01-24 [PMID: 23358892]

Bioinformatics

• Gene Symbol: DHCR24
• Entrez:
  • 1718(Human)
• Uniprot:
  • NP_055577.1(Human)