Secretagogin Antibody [Unconjugated]

Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Secretagogin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4878) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Secretagogin at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line and human pancreas tissue, loaded at 0.2 mg/mL. A specific band was detected for Secretagogin at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Secretagogin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4878) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Secretagogin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Secretagogin
  Asn164-Pro276
  Accession # O76038
• Specificity: Detects mouse Secretagogin in Western blots. Reactivity with human, rat, and other species is expected due to the high sequence homology of the immunogen used.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SCGN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Secretagogin Antibody [Unconjugated]

• CALBL
• DJ501N12.8
• SCGN
• SECRET
• Secretagogin
• secretagogin, EF-hand calcium binding protein
• SECRETsecretagogin
• SEGN
• Setagin

Background

Secretagogin (SCGN) is a 32 kDa member of the EF-hand family of Ca-binding proteins. It is expressed in select neurons, as well as neuroendocrine cells and pancreatic islets. Human Secretagogin is 276 amino acids (aa) in length and contains six 35 aa EF-hand domains (aa 12‑276). There is one alternative splice variant that shows a 22 aa substitution for aa 28‑276, and which demonstrates no Ca-binding ability. Secretagogin is known to circulate, presumably released during cell apoptosis. Over aa 164‑276, human Secretagogin is more than 97% aa identical to mouse, porcine and canine Secretagogin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Secretagogin Antibody (AF4878)(7)

Publications using AF4878

• Z Hevesi, D Zelena, RA Romanov, J Hanics, A Ignácz, A Zambon, DD Pollak, D Lendvai, K Schlett, M Palkovits, T Harkany, TGM Hökfelt, A Alpár Secretagogin marks amygdaloid PKC&delta interneurons and modulates NMDA receptor availability Proceedings of the National Academy of Sciences of the United States of America, 2021-02-16;118(7):. 2021-02-16 [PMID: 33558223] (Immunoprecipitation, Rat)
• Salib M, Joshi A, Katona L et al. GABAergic medial septal neurons with low-rhythmic firing innervating the dentate gyrus and hippocampal area CA3. J Neurosci. 3/29/2019 [PMID: 30926750]
• Alán Alpár, Péter Zahola, János Hanics, Zsófia Hevesi, Solomiia Korchynska, Marco Benevento et al. Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress The EMBO Journal 11/2/2018 [PMID: 30209240]
• Farid N. Garas, Eszter Kormann, Rahul S. Shah, Federica Vinciati, Yoland Smith, Peter J. Magill et al. Structural and molecular heterogeneity of calretinin-expressing interneurons in the rodent and primate striatum Journal of Comparative Neurology 4/1/2018 [PMID: 29218729]
• Farid N Garas, Rahul S Shah, Eszter Kormann, Natalie M Doig, Federica Vinciati, Kouichi C Nakamura et al. Secretagogin expression delineates functionally-specialized populations of striatal parvalbumin-containing interneurons eLife 9/26/2016 [PMID: 27669410]
• Adam C. Light, Yongling Zhu, Jun Shi, Shannon Saszik, Sarah Lindstrom, Laura Davidson et al. Organizational motifs for ground squirrel cone bipolar cells The Journal of Comparative Neurology 9/1/2012 [PMID: 22778006]
• Mulder J, Spence L, Tortoriello G, Dinieri JA, Uhlen M, Shui B, Kotlikoff MI, Yanagawa Y, Aujard F, Hokfelt T, Hurd YL, Harkany T Secretagogin is a Ca2+-binding protein identifying prospective extended amygdala neurons in the developing mammalian telencephalon. Eur. J. Neurosci., 2010-06-07;31(12):2166-77. 2010-06-07 [PMID: 20529129] (IHC-Fr, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: SCGN
• Uniprot:
  • O76038(Human)