SEC16A Antibody

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Independent Antibodies: Western Blot: SEC16A Antibody [NB100-1799] - Detection of Human KIAA0310 on HeLa whole cell lysate using NB100-1799. KIAA0310 was also immunoprecipitated by 2 other antibodies.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

SEC16A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human KIAA0310 using the numbering given in entry O15027 (GeneID 9919).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEC16A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1-4 ug/mg of lysate
Application Notes
ICC/IF reactivity reported in (PMID: 22740409).
Control
SEC16A Knockout HeLa Cell Lysate
Publications
Read Publications using
NB100-1799 in the following applications:

Reactivity Notes

Primate reactivity reported in (PMID: 22875974), Rat reactivity reported in (PMID: 22740409). Mouse reactivity reported in scientific literature (DOI 10.21007/etd.cghs.2016.0419).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SEC16A Antibody

  • FLJ26737
  • KIAA0310RP11-413M3.10
  • p250protein SEC16 homolog A
  • SEC16 homolog A (S. cerevisiae)
  • SEC16 homolog A
  • SEC16
  • SEC16Lprotein transport protein Sec16A

Background

KIAA0310 was first identified as a hypothetical protein predicted from the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. KIAA0310 appears to be the human Sec16 homolog. In budding yeast, Sec16 is an endoplasmic reticulum membrane protein that functions in vesicle transport through an interaction with COPII. Alternate names for KIAA0310 include SEC16 homolog A, SEC16A, SEC16L, p250, and RP11-413M.10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IP

Publications for SEC16A Antibody (NB100-1799)(11)

We have publications tested in 3 confirmed species: Mouse, Rat, Primate.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
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Filter By Species
Mouse
(1)
Rat
(1)
Primate
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-1799 Applications Species
Baird NL, York J, Nunberg JH. Arenavirus infection induces discrete cytosolic structures for RNA replication J Virol 2012 Aug 8 [PMID: 22875974] (Primate) Primate
van Zuylen WJ, Doyon P, Clement JF et al. Proteomic profiling of the TRAF3 interactome network reveals a new role for the ER-to-Golgi transport compartments in innate immunity. PLoS Pathog 2012 [PMID: 22792062]
Trahey M, Oh HS, Cameron CE, Hay JC. Poliovirus infection transiently increases COPII vesicle budding J Virol 2012 Jun 27 [PMID: 22740409] (ICC/IF, Rat) ICC/IF Rat
Zacharogianni M, Kondylis V, Tang Y et al. ERK7 is a negative regulator of protein secretion in response to amino-acid starvation by modulating Sec16 membrane association. EMBO J 2011 Aug [PMID: 21847093]
Smith JL, McBride CM, Nataraj PS et al. Trafficking-deficient hERG K+channels linked to long QT syndrome are regulated by a microtubule-dependent quality control compartment in the ER. Am J Physiol Cell Physiol 2011 Jul [PMID: 21490315]
Hughes H, Stephens DJ. Sec16A defines the site for vesicle budding from the endoplasmic reticulum on exit from mitosis. J Cell Sci 2010 Dec [PMID: 21045114]
Hughes H, Budnik A, Schmidt K et al. Organisation of human ER-exit sites: requirements for the localisation of Sec16 to transitional ER. J Cell Sci 2009 Aug [PMID: 19638414]
Townley AK, Feng Y, Schmidt K et al. Efficient coupling of Sec23-Sec24 to Sec13-Sec31 drives COPII-dependent collagen secretion and is essential for normal craniofacial development. J Cell Sci 2008 Sep [PMID: 18713835]
Abrami L, Kunz B, Iacovache I et al. Palmitoylation and ubiquitination regulate exit of the Wnt signaling protein LRP6 from the endoplasmic reticulum. Proc Natl Acad Sci U S A 2008 Apr [PMID: 18378904]
Wang B. Dissecting the Physiological Roles of ULK1/2 in the Mouse Brain Thesis. 2016 (Mouse) Mouse
Show All 11 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SEC16A Antibody (NB100-1799) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SEC16A
Entrez
Uniprot
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