![Western Blot: SEC13 Antibody (1280B) [MAB90551] - Analysis of HeLa, A431, and HepG2 cell lysates with 0.1 ug/ml of hSEC13 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hSEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize2/SEC13-Antibody-(1280B)-Western-Blot-MAB90551-img0001.jpg "Western Blot: SEC13 Antibody (1280B) [MAB90551] - Analysis of HeLa, A431, and HepG2 cell lysates with 0.1 ug/ml of hSEC13 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hSEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clone | 1280B |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | rhSEC13 aa123-322 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SEC13 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C in the dark. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | MAB90551AF350 | |
| Alexa Fluor 405 | MAB90551AF405 | |
| Alexa Fluor 488 | MAB90551AF488 | |
| Alexa Fluor 532 | MAB90551AF532 | |
| Alexa Fluor 594 | MAB90551AF594 | |
| Alexa Fluor 647 | MAB90551AF647 | |
| Alexa Fluor 700 | MAB90551AF700 | |
| Alexa Fluor 750 | MAB90551AF750 | |
| Allophycocyanin | MAB90551APC | |
| Biotin | MAB90551B | |
| DyLight 350 | MAB90551UV | |
| DyLight 405 | MAB90551V | |
| DyLight 488 | MAB90551G | |
| DyLight 550 | MAB90551R | |
| DyLight 594 | MAB90551DL594 | |
| DyLight 650 | MAB90551C | |
| DyLight 680 | MAB90551FR | |
| DyLight 755 | MAB90551IR | |
| FITC | MAB90551F | |
| HRP | MAB90551H | |
| PE | MAB90551PE |
Diseases for SEC13 Antibody (MAB90551)Discover more about diseases related to SEC13 Antibody (MAB90551).
| Pathways for SEC13 Antibody (MAB90551)View related products by pathway.
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PTMs for SEC13 Antibody (MAB90551)Learn more about PTMs related to SEC13 Antibody (MAB90551).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SEC13 |
![Western Blot: SAR1 Antibody [NBP1-80789] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SAR1A antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize2/SAR1-Antibody-Western-Blot-NBP1-80789-img0008.jpg)
![Immunohistochemistry-Paraffin: SAR1 Antibody [NBP1-80789] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/SAR1-Antibody-Immunohistochemistry-Paraffin-NBP1-80789-img0002.jpg)
![Western Blot: SAR1 Antibody [NBP1-80789] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize2/SAR1-Antibody-Western-Blot-NBP1-80789-img0007.jpg)
![Western Blot: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of IQGAP1 on different lysates using anti-IQGAP1 antibody at 1/1,000 dilution.Positive control:Lane 1: HUVEC Lane 2: A431Lane 3: Mouse placenta](http://images.novusbio.com/fullsize2/IQGAP1 Antibody (JG38-74)-Western Blot-NBP2-75545-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Staining IQGAP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/IQGAP1-Antibody-(JG38-74)-Immunocytochemistry-Immunofluorescence-NBP2-75545-img0004.jpg)
![Immunohistochemistry-Paraffin: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of paraffin-embedded mouse colon tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/IQGAP1-Antibody-(JG38-74)-Immunohistochemistry-Paraffin-NBP2-75545-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SEC24B Antibody [NBP1-86263] - Staining of human cell line A-431 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/SEC24B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86263-img0004.jpg)
![Immunohistochemistry-Paraffin: SEC24B Antibody [NBP1-86263] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize2/SEC24B Antibody-Immunohistochemistry-Paraffin-NBP1-86263-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SEC31A Antibody [NBP1-89301] - Staining of human cell line U-251 MG shows localization to cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/SEC31A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89301-img0006.jpg)
![Immunohistochemistry-Paraffin: SEC31A Antibody [NBP1-89301] - Staining of human skeletal muscle shows very weak positivity in myocytes.](http://images.novusbio.com/fullsize2/SEC31A-Antibody-Immunohistochemistry-Paraffin-NBP1-89301-img0016.jpg)
![Immunohistochemistry-Paraffin: SEC31A Antibody [NBP1-89301] - Staining of human cerebral cortex shows positivity in neurons.](http://images.novusbio.com/fullsize2/SEC31A-Antibody-Immunohistochemistry-Paraffin-NBP1-89301-img0013.jpg)
![Western Blot: SEC16A Antibody [NB100-1799] - Detection of Human KIAA0310 on HeLa whole cell lysate using NB100-1799. KIAA0310 was also immunoprecipitated by 2 other antibodies.](http://images.novusbio.com/fullsize2/SEC16A-Antibody-Western-Blot-NB100-1799-img0002.jpg)
![Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize2/Niemann-Pick-C1-Antibody-Knockout-Validated-NB400-148-img0014.jpg)
![Western Blot: Niemann-Pick C1 Antibody [NB400-148] - NPC proteins in mouse lungs. Western blot of wild type (W) littermates, NPC1 (Mut) or NPC2 (Mut) mutant mouse lungs using anti-NPC1 or -NPC2 antibody. beta-actin used as a loading control. 30 ug protein/lane. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0067084), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Niemann-Pick C1 Antibody-Western Blot-NB400-148-img0015.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody [NB400-148] - Staining of human brain, cortex, neurons and astrocytes.](http://images.novusbio.com/fullsize2/Niemann-Pick-C1-Antibody-Immunohistochemistry-Paraffin-NB400-148-img0011.jpg)
![Western Blot: SEC23A Antibody [NBP2-34842] - Analysis was performed whole cell extracts and tissue extracts of HeLa (Lane 1), PC-12 (Lane 2), NIH/3T3 (Lane 3), Mouse Liver (Lane 4) and Rat Liver (Lane 5).](http://images.novusbio.com/fullsize2/SEC23A-Antibody-Western-Blot-NBP2-34842-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SEC23A Antibody [NBP2-34842] - Analysis of COP II (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize2/SEC23A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34842-img0002.jpg)
![Flow Cytometry: SEC23A Antibody [NBP2-34842] - Analysis of COP II was done on PC-12 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with COP II Rabbit Polyclonal Antibody (PA1-069A, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA.](http://images.novusbio.com/fullsize2/SEC23A-Antibody-Flow-Cytometry-NBP2-34842-img0006.jpg)
![Western Blot: SEC23IP Antibody [NBP1-82456] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/SEC23IP-Antibody-Western-Blot-NBP1-82456-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SEC23IP Antibody [NBP1-82456] - Staining of human cell line A-431 shows positivity in vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/SEC23IP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82456-img0006.jpg)
![Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/SEC23IP-Antibody-Immunohistochemistry-Paraffin-NBP1-82456-img0010.jpg)
![Western Blot: Nucleoporin 107 Antibody [NBP1-76926] - A549 cell lysate with NUP107 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize2/Nucleoporin-107-Antibody-Western-Blot-NBP1-76926-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleoporin 107 Antibody [NBP1-76926] - Immunofluorescence of NUP107 in A549 cells with NUP107 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize2/Nucleoporin-107-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76926-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleoporin 107 Antibody [NBP1-76926] - A549 cells with Nucleoporin 107 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize2/Nucleoporin-107-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76926-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: COP Antibody [NB100-856] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize2/COP-Antibody-Immunocytochemistry-Immunofluorescence-NB100-856-img0001.jpg)
![Flow Cytometry: COP Antibody [NB100-856] - Analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize2/COP-Antibody-Flow-Cytometry-NB100-856-img0002.jpg)
![Western Blot: ATP7b Antibody [NB100-360] - ATP7b detected in 20 ug of mouse brain membrane fraction.](http://images.novusbio.com/fullsize2/ATP7b Antibody-Western Blot-NB100-360-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NB100-360] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ATP7b [NB100-360] at a 1:100 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/ATP7b-Antibody-Immunocytochemistry-Immunofluorescence-NB100-360-img0005.jpg)
![Immunohistochemistry: ATP7b Antibody [NB100-360] - Analysis of ATP7b in mouse liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/ATP7b-Antibody-Immunohistochemistry-NB100-360-img0004.jpg)
![Western Blot: SAR1B Antibody [NBP1-32725] - Adult mouse small intestine homogenate, 20 ug total protein per lane. Antibody at 1:1000. Secondary HRP conjugated antibody at 1:5000. Bands visualized with Immobilon Western Chemiluminescence HRP substrate, imaged with Syngene system. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/SAR1B-Antibody-Western-Blot-NBP1-32725-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SAR1B Antibody [NBP1-32725] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize2/SAR1B Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32725-img0005.jpg)
![Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725] - Mouse ovary. SAR1B antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize2/SAR1B-Antibody-Immunohistochemistry-Paraffin-NBP1-32725-img0010.jpg)
![Western Blot: NUP98 Antibody [NB100-93325] - Detection of Human NUP96 on HeLa whole cell lysate using NB100-93325. NUP96 was also immunoprecipitated by rabbit anti-NUP96 antibody NB100-93326.](http://images.novusbio.com/fullsize2/NUP98-Antibody-Western-Blot-NB100-93325-img0002.jpg)
![Immunohistochemistry-Paraffin: SEH1L Antibody [NBP1-80773] - Staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/SEH1L-Antibody-Immunohistochemistry-Paraffin-NBP1-80773-img0005.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")